Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD45R antibody [RA3-6B2]

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Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lymph node)
Specification
Lymph node
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH6.0
Permeabilization
No
Blocking step
BSA as blocking agent for 25 minute(s) · Concentration: 1% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA/TPBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti- rat Polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Here shows one of the lymphoid follicle of murine lymph node.
Small lymphocytes in cortical area were CD45R strong positive, while large lymphocytes in germinal center weak positive. CD45R positive cells are B-lymphocytes at different stage of maturation/differentiation.

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Submitted Jan 13 2011

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