Overview

  • Product name
    Anti-CD45RA antibody [B-C15] (FITC)
    See all CD45RA primary antibodies
  • Description
    Mouse monoclonal [B-C15] to CD45RA (FITC)
  • Host species
    Mouse
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody is specific for CD45RA (with different isoforms of 205/220 kDa).
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Reh 6 cell line

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

     

Properties

Applications

Our Abpromise guarantee covers the use of ab27290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10 µl to label 106 cells or 100 µl of whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
    • Involvement in disease
      Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
      Multiple sclerosis
    • Sequence similarities
      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.
    • Domain
      The first PTPase domain interacts with SKAP1.
    • Post-translational
      modifications
      Heavily N- and O-glycosylated.
    • Cellular localization
      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • B220 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • Loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • Protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Images

    • A typical staining pattern with ab27290.

    References

    This product has been referenced in:
    • McRae BL  et al. Interferon-alpha and -beta inhibit the in vitro differentiation of immunocompetent human dendritic cells from CD14(+) precursors. Blood 96:210-7 (2000). Read more (PubMed: 10891453) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your patience in this matter. In regards to ab30476 (CD45RA antibody [F8-11-13] (Biotin)), the originator does not know which epitope this antibody is recognizing. However, information available in the literature suggests that it recognizes a 'restricted' epitope present on B lymphocytes and a sub-population of T lymphocytes. Please see the following articles for further information: Dalchau R, Fabre JW. Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence or structural and possible functional diversity of the human leukocyte common molecule. J Exp Med. 1981 Apr 1;153(4):753-65. PMID: 6166706 Ritter MA, Sauvage CA, Pegram SM, Myers CD, Dalchau R, Fabre JW. The human leucocyte-common (LC) molecule: dissection of leukaemias using monoclonal antibodies directed against framework and restricted antigenic determinants. Leuk Res. 1985;9(10):1249-54. PMID: 2415779 Although ab8217 (CD45RA antibody [MEM-56]) has not been epitope mapped, the originator thinks it is very probable that this antibody could be recognizing a glycosylated epitope. Regarding ab27290 (CD45RA antibody [B-C15] (FITC)) and ab38439 (CD45RA antibody [LT45-M5]), the originators do not have any information on the epitopes that the antibodies are recognizing. I hope this information helps.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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