Anti-CD45RA antibody [MEM-56] (ab8217)

Submit a review Q&A (1)References (3)

Overview

  • Product name

    Anti-CD45RA antibody [MEM-56]
    See all CD45RA primary antibodies

  • Description

    Mouse monoclonal [MEM-56] to CD45RA

  • Host species

    Mouse

  • Specificity

    CD45RA antigen (human)

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue/ cell preparation (Human). (Thymocytes and T lymphocytes).

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab8217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
WB Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.
Flow Cyt Use a concentration of 1 - 4 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.

Target

  • Function

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

  • Involvement in disease

    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
    Multiple sclerosis

  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.

  • Domain

    The first PTPase domain interacts with SKAP1.

  • Post-translational
    modifications

    Heavily N- and O-glycosylated.

  • Cellular localization

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • Database links

    • Alternative names

      • B220 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • Loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • Protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    Images

    • Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)
      Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)
      Lane 1 : CD45 control antibody
      Lanes 2-3 : Anti-CD45RA antibody [MEM-56] (ab8217)

      Lane 1 : HUT-78 human cutaneous T cell lymphoma cell line.
      Lane 2 : sample of cell lysate prepared under non-reducing conditions
      Lane 3 : sample of cell lysate prepared under reducing conditions

    References

    This product has been referenced in:

    • Abdulamir AS  et al. Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma. BMC Immunol 9:73 (2008). ICC ; Human . Read more (PubMed: 19087256) »
    • Bazil V  et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). Read more (PubMed: 2534480) »
    See all 3 Publications for this product

    Publishing research using ab8217? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    ab8217 CD45RA antibody [MEM-56] ab27290 CD45RA antibody [B-C15] (FITC) ab30476 CD45RA antibody [F8-11-13] ab38439 CD45RA antibody [LT45-M5] What epitopes do these antibodies recognize; do they recognize glycosylated dependent epitopes?

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    Answer

    Thank you for your patience in this matter. In regards to ab30476 (CD45RA antibody [F8-11-13] (Biotin)), the originator does not know which epitope this antibody is recognizing. However, information available in the literature suggests that it recognizes a 'restricted' epitope present on B lymphocytes and a sub-population of T lymphocytes. Please see the following articles for further information: Dalchau R, Fabre JW. Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence or structural and possible functional diversity of the human leukocyte common molecule. J Exp Med. 1981 Apr 1;153(4):753-65. PMID: 6166706 Ritter MA, Sauvage CA, Pegram SM, Myers CD, Dalchau R, Fabre JW. The human leucocyte-common (LC) molecule: dissection of leukaemias using monoclonal antibodies directed against framework and restricted antigenic determinants. Leuk Res. 1985;9(10):1249-54. PMID: 2415779 Although ab8217 (CD45RA antibody [MEM-56]) has not been epitope mapped, the originator thinks it is very probable that this antibody could be recognizing a glycosylated epitope. Regarding ab27290 (CD45RA antibody [B-C15] (FITC)) and ab38439 (CD45RA antibody [LT45-M5]), the originators do not have any information on the epitopes that the antibodies are recognizing. I hope this information helps.

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