Anti-CD45RA antibody [MEM-56] (ab8217)

Submit a review Q&A (1)References (4)
Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)

    Key features and details

    • Mouse monoclonal [MEM-56] to CD45RA
    • Suitable for: WB, IHC-P
    • Reacts with: Human
    • Isotype: IgG2b

    Overview

    • Product name

      Anti-CD45RA antibody [MEM-56]
      See all CD45RA primary antibodies

    • Description

      Mouse monoclonal [MEM-56] to CD45RA

    • Host species

      Mouse

    • Specificity

      CD45RA antigen (human)

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Tissue/ cell preparation (Human). (Thymocytes and T lymphocytes).

    • Positive control

      • WB: HUT-78 human cutaneous T cell lymphoma cell lysate.

    • General notes

      This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab8217 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.
    IHC-P
    Use at an assay dependent concentration.
    Notes
    WB
    Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.
    IHC-P
    Use at an assay dependent concentration.

    Target

    • Function

      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

    • Involvement in disease

      Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
      Multiple sclerosis

    • Sequence similarities

      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.

    • Domain

      The first PTPase domain interacts with SKAP1.

    • Post-translational
      modifications

      Heavily N- and O-glycosylated.

    • Cellular localization

      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt

    • Database links

      • Alternative names

        • B220 antibody
        • CD45 antibody
        • CD45 antigen antibody
        • CD45R antibody
        • EC 3.1.3.48 antibody
        • GP180 antibody
        • L-CA antibody
        • LCA antibody
        • Leukocyte common antigen antibody
        • Loc antibody
        • Ly-5 antibody
        • LY5 antibody
        • Ly5, homolog of antibody
        • Lymphocyte antigen 5 antibody
        • Lyt-4 antibody
        • Protein tyrosine phosphatase, receptor type C antibody
        • Protein tyrosine phosphatase, receptor type, C antibody
        • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
        • Ptprc antibody
        • PTPRC_HUMAN antibody
        • Receptor-type tyrosine-protein phosphatase C antibody
        • RT7 antibody
        • T200 antibody
        • T200 glycoprotein antibody
        • T200 leukocyte common antigen antibody
        see all

      Images

      • Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)
        Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)
        Lane 1 : CD45 control antibody
        Lanes 2-3 : Anti-CD45RA antibody [MEM-56] (ab8217)

        Lane 1 : HUT-78 human cutaneous T cell lymphoma cell line.
        Lane 2 : sample of cell lysate prepared under non-reducing conditions
        Lane 3 : sample of cell lysate prepared under reducing conditions

      References (4)

      Publishing research using ab8217? Please let us know so that we can cite the reference in this datasheet.

      ab8217 has been referenced in 4 publications.

      • Zizzari IG  et al. The Macrophage Galactose-Type C-Type Lectin (MGL) Modulates Regulatory T Cell Functions. PLoS One 10:e0132617 (2015). PubMed: 26147970
      • Abdulamir AS  et al. Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma. BMC Immunol 9:73 (2008). ICC ; Human . PubMed: 19087256
      • Bazil V  et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480
      • Bazil V  et al. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. Immunogenetics 29:202-5 (1989). PubMed: 2466778

      Customer reviews and Q&As

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      Question

      ab8217 CD45RA antibody [MEM-56] ab27290 CD45RA antibody [B-C15] (FITC) ab30476 CD45RA antibody [F8-11-13] ab38439 CD45RA antibody [LT45-M5] What epitopes do these antibodies recognize; do they recognize glycosylated dependent epitopes?

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      Answer

      Thank you for your patience in this matter. In regards to ab30476 (CD45RA antibody [F8-11-13] (Biotin)), the originator does not know which epitope this antibody is recognizing. However, information available in the literature suggests that it recognizes a 'restricted' epitope present on B lymphocytes and a sub-population of T lymphocytes. Please see the following articles for further information: Dalchau R, Fabre JW. Identification with a monoclonal antibody of a predominantly B lymphocyte-specific determinant of the human leukocyte common antigen. Evidence or structural and possible functional diversity of the human leukocyte common molecule. J Exp Med. 1981 Apr 1;153(4):753-65. PMID: 6166706 Ritter MA, Sauvage CA, Pegram SM, Myers CD, Dalchau R, Fabre JW. The human leucocyte-common (LC) molecule: dissection of leukaemias using monoclonal antibodies directed against framework and restricted antigenic determinants. Leuk Res. 1985;9(10):1249-54. PMID: 2415779 Although ab8217 (CD45RA antibody [MEM-56]) has not been epitope mapped, the originator thinks it is very probable that this antibody could be recognizing a glycosylated epitope. Regarding ab27290 (CD45RA antibody [B-C15] (FITC)) and ab38439 (CD45RA antibody [LT45-M5]), the originators do not have any information on the epitopes that the antibodies are recognizing. I hope this information helps.

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