Anti-CD45RA antibody [MEM-56] (ab8217)
Key features and details
- Mouse monoclonal [MEM-56] to CD45RA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-CD45RA antibody [MEM-56]
See all CD45RA primary antibodies -
Description
Mouse monoclonal [MEM-56] to CD45RA -
Host species
Mouse -
Specificity
CD45RA antigen (human) -
Tested Applications & Species
Application Species WB Human -
Immunogen
Tissue/ cell preparation (Human). (Thymocytes and T lymphocytes).
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Positive control
- WB: HUT-78 human cutaneous T cell lymphoma cell lysate.
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General notes
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
MEM-56 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
Involvement in disease
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
Images
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Lane 1 : CD45 control antibody
Lanes 2-3 : Anti-CD45RA antibody [MEM-56] (ab8217)
Lane 1 : HUT-78 human cutaneous T cell lymphoma cell line.
Lane 2 : sample of cell lysate prepared under non-reducing conditions
Lane 3 : sample of cell lysate prepared under reducing conditions
References (4)
ab8217 has been referenced in 4 publications.
- Zizzari IG et al. The Macrophage Galactose-Type C-Type Lectin (MGL) Modulates Regulatory T Cell Functions. PLoS One 10:e0132617 (2015). PubMed: 26147970
- Abdulamir AS et al. Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma. BMC Immunol 9:73 (2008). ICC ; Human . PubMed: 19087256
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480
- Bazil V et al. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. Immunogenetics 29:202-5 (1989). PubMed: 2466778