Overview

  • Product name

    Anti-CD45RA antibody [SPM504]
    See all CD45RA primary antibodies
  • Description

    Mouse monoclonal [SPM504] to CD45RA
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD45RA. (Stimulated human leucocytes).

  • Positive control

    • IHC-P: Human tonsil, spleen, thymus, stomach, colon, B cell lymphoma, HK lymphoma, esophagus and uterus tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab231426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.

No antigen retreival necessary. Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • Involvement in disease

    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
    Multiple sclerosis
  • Sequence similarities

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • Domain

    The first PTPase domain interacts with SKAP1.
  • Post-translational
    modifications

    Heavily N- and O-glycosylated.
  • Cellular localization

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • Database links

  • Alternative names

    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • EC 3.1.3.48 antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • Loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lymphocyte antigen 5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase, receptor type C antibody
    • Protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • RT7 antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human B cell lymphoma tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human esophagus tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human uterus tissue stained for CD45RA using ab231426 at 1/200 dilution in immunohistochemical analysis.

References

ab231426 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted May 16 2019

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