Anti-CD45RB antibody [MEM-55] (ab8218)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-CD45RB antibody [MEM-55]
See all CD45RB primary antibodies -
Description
Mouse monoclonal [MEM-55] to CD45RB -
Host species
Mouse -
Specificity
CD45RB antigen (human). This antibody recognizes a siliadase-sensitive epitope expressed on most PBL. -
Tested applications
Suitable for: WB, IP, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD45RB.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
MEM-55 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab8218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. | |
IHC-P | Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. | |
Flow Cyt | Use a concentration of 2 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
Protocols
References
This product has been referenced in:
- Bazil V et al. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. Immunogenetics 29:202-5 (1989). Read more (PubMed: 2466778) »
- Knapp W et al. Leucocyte typing IV. Oxford University Press : (1989). Read more (PubMed: ) »