Overview

  • Product name
    Anti-CD45RC antibody [IBL-8]
    See all CD45RC primary antibodies
  • Description
    Rat monoclonal [IBL-8] to CD45RC
  • Host species
    Rat
  • Specificity
    This antibody recognises the exon C-dependent RC isoform of CD45. It reacts strongly with B cells and less intensely with most CD8+ T cells. It does not recognise CD4+ T cells.
  • Tested applications
    Suitable for: Flow Cyt, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
  • Cellular localization
    Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
  • Database links
  • Alternative names
    • B220 antibody
    • CD45 antibody
    • CD45R antibody
    • EC 3.1.3.48 antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lymphocyte antigen 5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase receptor type C antibody
    • PTPRC antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • RT7 antibody
    • T200 antibody
    • T200 Glycoprotein antibody
    see all

Images

  • Immunohistochemistry of mouse spleen staining CD45RC using Anti-CD45RC antibody [IBL-8] (ab3135)

     

References

This product has been referenced in:
  • Zhao HY  et al. Prognostic significance of thymidylate synthase in postoperative non-small cell lung cancer patients. Onco Targets Ther 7:1301-10 (2014). IHC . Read more (PubMed: 25114572) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.

I am sorry for the delay in my answer. I was waiting for an answer from the sources of these antibodies.

I can confirm that both antibodies were not tested from the lab with other CD45 isoforms.

ab3135 was not tested on all of CD45 subtypes because its reactivity is corresponding to the CD45 (LCA) phenotype.

ab112171: The original characterization of the clone was done by Bottomly et al (1989):

Bottomly, K., M. Lugman, L. Greenbaum, S. Carding, I. West, T. Pasqualini, and D.B. Murphy. A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines. Eur. J. Immunol. 1989. 19:617-623.


The following publication also might be interesting for you:

Hathcock, K.S., H. Hirano, R.J. Hodes. CD45 expression by murine B cells and T cells: Alteration of CD45 isoforms in subpopulations of activated B cells. Immunol. Res. 1993. 12:21-36.

Hathcock, K.S et al (1992). Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations. Journal of Immunology, 148(1), p 19-28



I hope this information is helpful and wish you good luck with your experiments.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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