Anti-CD45RC antibody [MIL15] (ab14125)
![Flow Cytometry - Anti-CD45RC antibody [MIL15] (ab14125)](https://www.abcam.com/ps/products/14/ab14125/Images/ab14125-448502-anti-cd45rc-antibody-mil15-flow-cytometry-mononuclear-cells-pig.jpg "Flow Cytometry - Anti-CD45RC antibody [MIL15] (ab14125)")
Key features and details
- Mouse monoclonal [MIL15] to CD45RC
- Suitable for: Flow Cyt
- Reacts with: Pig
- Isotype: IgM
Overview
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Product name
Anti-CD45RC antibody [MIL15]
See all CD45RC primary antibodies -
Description
Mouse monoclonal [MIL15] to CD45RC -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Pig -
Immunogen
Full length native protein (purified) (Pig). (Cells isolated from porcine mesenteric lymph node).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: Tissue culture supernatant, 1.72% Tris HCl, 10% Fetal calf serum -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
MIL15 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab14125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 10µl for 106 cells. |
Target
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Relevance
The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform. -
Cellular localization
Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts. -
Alternative names
- B220 antibody
- CD45 antibody
- CD45R antibody
see all
Images
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Flow Cytometry - Anti-CD45RC antibody [MIL15] (ab14125)
Figure A. FITC conjugated mouse anti porcine CD3 and purified mouse IgM isotype control detected with goat anti mouse IgM PE.
Figure B. FITC conjugated mouse anti porcine CD3 and purified mouse Anti-CD45RC antibody [MIL15] (ab14125) detected with goat anti mouse IgM.
All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Datasheets and documents
References (0)
ab14125 has not yet been referenced specifically in any publications.
