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    cd45rc-antibody-mil15-ab14125.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD45RC antibody [MIL15] (ab14125)

  • Datasheet
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Flow Cytometry - Anti-CD45RC antibody [MIL15] (ab14125)

    Key features and details

    • Mouse monoclonal [MIL15] to CD45RC
    • Suitable for: Flow Cyt
    • Reacts with: Pig
    • Isotype: IgM

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    Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
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    APC Anti-CD45RC antibody [RP1/12] (ab91011)

    View more associated products

    Overview

    • Product name

      Anti-CD45RC antibody [MIL15]
      See all CD45RC primary antibodies
    • Description

      Mouse monoclonal [MIL15] to CD45RC
    • Host species

      Mouse
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Pig
    • Immunogen

      Full length native protein (purified) (Pig). (Cells isolated from porcine mesenteric lymph node).

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituents: Tissue culture supernatant, 1.72% Tris HCl, 10% Fetal calf serum
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      MIL15
    • Isotype

      IgM
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Phosphatases
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
      • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
      • Goat Anti-Mouse IgM H&L (ab9167)
      • Rabbit Anti-Mouse IgM mu chain (ab9175)
    • Isotype control

      • Mouse IgM [B11/7] - Isotype control (ab91545)

    Applications

    Our Abpromise guarantee covers the use of ab14125 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    Target

    • Relevance

      The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
    • Cellular localization

      Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
    • Alternative names

      • B220 antibody
      • CD45 antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase receptor type C antibody
      • PTPRC antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 Glycoprotein antibody
      see all

    Images

    • Flow Cytometry - Anti-CD45RC antibody [MIL15] (ab14125)
      Flow Cytometry - Anti-CD45RC antibody [MIL15] (ab14125)

      Figure A. FITC conjugated mouse anti porcine CD3 and purified mouse IgM isotype control detected with goat anti mouse IgM PE.

      Figure B. FITC conjugated mouse anti porcine CD3 and purified mouse Anti-CD45RC antibody [MIL15] (ab14125) detected with goat anti mouse IgM.

      All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab14125? Please let us know so that we can cite the reference in this datasheet.

    ab14125 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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