- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CD45RC antibody [MIL15]
See all CD45RC primary antibodies -
DescriptionMouse monoclonal [MIL15] to CD45RC
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Host speciesMouse
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Tested applicationsSuitable for: IHC-Fr, IHC-P, Flow Cytmore details
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Species reactivityReacts with: Pig
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Immunogen
Full length native protein (purified) (Pig). (Cells isolated from porcine mesenteric lymph node).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.4
Preservative: 0.09% Sodium azide
Constituents: Tissue culture supernatant, 1.72% Tris HCl, 10% Fetal calf serum -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberMIL15
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IsotypeIgM
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab14125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | ||
| IHC-P | ||
| Flow Cyt |
Application notes
FACS: Use 10µl for 106 cells.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceThe CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
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Cellular localizationMembrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
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Alternative names
- B220 antibody
- CD45 antibody
- CD45R antibody
see all
References
ab14125 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"