Overview

  • Product name
    Anti-CD45RC antibody [MRC OX-22]
    See all CD45RC primary antibodies
  • Description
    Mouse monoclonal [MRC OX-22] to CD45RC
  • Host species
    Mouse
  • Specificity
    ab33945 recognises rat CD45RC, the high molecular weight form of the leucocyte common antigen. The antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations, CD4+CD45RChigh (Th1-like) and CD4+CD45RClow (Th2-like).
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    PHA stimulated rat lymphocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab33945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
IHC-P
  • Application notes
    Flow Cyt: 1/100.
    IHC-P: 1/100 - 1/1000. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    IHC-Fr: 1/100 - 1/1000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
    • Cellular localization
      Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
    • Database links
    • Alternative names
      • B220 antibody
      • CD45 antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase receptor type C antibody
      • PTPRC antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 Glycoprotein antibody
      see all

    References

    ab33945 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spleen, Lung)
    Specification
    Spleen, Lung
    Fixative
    10% NBF, 24 hours
    Antigen retrieval step
    Enzymatic

    Abcam user community

    Verified customer

    Submitted Jul 11 2012

    Question
    Answer

    Thank you for your recent telephone enquiry. I have obtained the following general testing method from the originator of ab33933 and ab33934. Please note that this protocol is a guideline only and may need to be optimized in your laboraotry. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us. 1. De-paraffinise sections thoroughly in Xylene/synthetic solvent and hydrate through graded series of alcohols. 2. Wash twice in TBS. 3. If required, treat with 0.3% (w/v) hydrogen peroxide in methanol for 30 minutes to block endogenous peroxidase activity (2% w/v H2O2/Methanol can be used for a shorter time if preferred). 4. Wash once in TBS. 5. If required, include appropriate antigen retrieval step to enhance immunostaining. 6. Wash once in TBS. 7. Incubate sections for 10 minutes in 10% normal serum from species in which secondary antibody was raised. 8. Tap excess serum off the slides before staining. 9. Incubate sections in primary antibody for at least 1 hour at room temperature in a humid chamber or overnight at 4°C. Wash three times in TBS. 10. Add enzyme conjugated secondary antibody at recommended dilution (see specific datasheet for details). Incubate for at least 30 minutes at room temperature. 11. Wash three times in TBS. 12. Incubate in appropriate substrate solution for recommended period of time (we would recommends the use of DAB substrate with HRP conjugated antibodies, and Fast Red / Napthol AS-MX for Alkaline Phosphatase conjugated antibodies). 13. Wash once in water. 14. Counterstain in haematoxylin, 2-10 minutes. ‘Blue’ in running water for 5 minutes. 15. Mount in aqueous mounting medium or alternatively dehydrate through alcohols and xylene/solvent and mount in synthetic mountant. Notes: NB. Note that certain substrates are soluble in alcohol - please refer to supplier information for details. Appropriate control samples should always be included. It may be useful to include a control in which no primary antibody is used at all, to determine any non-specific binding of the secondary reagent to the target tissue. Solutions used: TBS (stock solution ×10 concentrated) Sodium chloride 87.66 g, Tris 60.55 g, Distilled water 1 litre. Adjust pH to 7.5 using concentrated HCl.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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