Anti-CD45RC antibody [MRC OX-22] (Biotin) (ab33937)


  • Product name

    Anti-CD45RC antibody [MRC OX-22] (Biotin)
    See all CD45RC primary antibodies
  • Description

    Mouse monoclonal [MRC OX-22] to CD45RC (Biotin)
  • Host species

  • Conjugation

  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    PHA stimulated rat lymphocytes

  • Positive control

    • rat splenocytes.



Our Abpromise guarantee covers the use of ab33937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use neat. Use 10µl of the suggested working dilution to label 106 cells in 100µl.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.
    • Cellular localization

      Membrane; Single-pass type I membrane protein. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.
    • Database links

    • Alternative names

      • B220 antibody
      • CD45 antibody
      • CD45R antibody
      • EC antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase receptor type C antibody
      • PTPRC antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 Glycoprotein antibody
      see all


    • Flow Cytometry analysis of rat splenocytes labeling CD45RC with ab33937.


    ab33937 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A


    Thank you for contacting us.

    Our labs follow the suggested protocols posted within our Protocol and Troubleshooting Page at The indirect flow cytometry protocol may be accessed directly at this page: Within this protocol you will find that our recommended time and temperature for incubation of these antibodies is ˜30m minutes at room temperature. Please be aware that differences in cell type will require optimization of this protocol experimentally.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Thank you for contacting us.

    Optimal dilution for AB33937 from our QC testing is 1:2. We used 10ul of this working dilution to stain one million cells in 100ul total volume (an additional 1:10 dilution). For the streptavidin detection reagent, we used STAR4ASTREPTAVIDIN: RPE which is a PE conjugate. We used this undiluted (again with the 1:10 dilution which is part of our flow cytometry protocol).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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