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    cd45rc-antibody-mrc-ox-22-bsa-and-azide-free-ab244570.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (ab244570)

  • Datasheet
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Immunohistochemistry (Frozen sections) - Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (ab244570)

    Key features and details

    • Mouse monoclonal [MRC OX-22] to CD45RC - BSA and Azide free
    • Suitable for: IHC-Fr, IHC-P, Flow Cyt
    • Reacts with: Rat, Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

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    Overview

    • Product name

      Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free
      See all CD45RC primary antibodies
    • Description

      Mouse monoclonal [MRC OX-22] to CD45RC - BSA and Azide free
    • Host species

      Mouse
    • Tested applications

      Suitable for: IHC-Fr, IHC-P, Flow Cytmore details
    • Species reactivity

      Reacts with: Rat, Human
    • Immunogen

      Tissue, cells or virus corresponding to Rat CD45RC. PHA stimulated rat lymphocytes.

    • Positive control

      • IHC-Fr: Rat spleen tissue.
    • General notes

      ab244570 is the carrier-free version of ab33945.

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
    • Storage buffer

      Constituent: PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      MRC OX-22
    • Myeloma

      NS1
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • B Cells
      • CD
      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Phosphatases
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage

    Associated products

    • Alternative Versions

      • Alexa Fluor® 488 Anti-CD45RC antibody [MRC OX-22] (ab256260)
      • Anti-CD45RC antibody [MRC OX-22] (ab33945)
    • Conjugation kits

      • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab244570 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr
    Use a concentration of 1 µg/ml.

    This product gave a positive signal in HeLa cells fixed with 10% formaldehyde (10 min).

    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Flow Cyt
    Use at an assay dependent concentration.
    Notes
    IHC-Fr
    Use a concentration of 1 µg/ml.

    This product gave a positive signal in HeLa cells fixed with 10% formaldehyde (10 min).

    IHC-P
    Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Flow Cyt
    Use at an assay dependent concentration.

    Target

    • Function

      Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
    • Involvement in disease

      Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
      Multiple sclerosis
    • Sequence similarities

      Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 tyrosine-protein phosphatase domains.
    • Domain

      The first PTPase domain interacts with SKAP1.
    • Post-translational
      modifications

      Heavily N- and O-glycosylated.
    • Cellular localization

      Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Target information above from: UniProt accession P08575 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5788 Human
      • Entrez Gene: 24699 Rat
      • Omim: 151460 Human
      • SwissProt: P08575 Human
      • SwissProt: P04157 Rat
      • Unigene: 654514 Human
      • Unigene: 90166 Rat
      • Alternative names

        • B220 antibody
        • CD45 antibody
        • CD45R antibody
        • EC 3.1.3.48 antibody
        • GP180 antibody
        • L-CA antibody
        • LCA antibody
        • Leukocyte common antigen antibody
        • loc antibody
        • Ly-5 antibody
        • LY5 antibody
        • Ly5, homolog of antibody
        • Lymphocyte antigen 5 antibody
        • Lyt-4 antibody
        • Protein tyrosine phosphatase receptor type C antibody
        • Ptprc antibody
        • PTPRC_HUMAN antibody
        • Receptor-type tyrosine-protein phosphatase C antibody
        • RT7 antibody
        • T200 antibody
        • T200 Glycoprotein antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (ab244570)
        Immunohistochemistry (Frozen sections) - Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (ab244570)

        IHC image of CD45RC staining in a section of frozen normal rat spleen*.

        The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.

        Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab33945 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

        The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33945.

        For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

        *Tissue obtained from Charles River.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium Azide (ab33945)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (1)

      Publishing research using ab244570? Please let us know so that we can cite the reference in this datasheet.

      ab244570 has been referenced in 1 publication.

      • Spickett GP  et al. MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen. J Exp Med 158:795-810 (1983). PubMed: 6224884

      Customer reviews and Q&As

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