Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (ab244570)
![Immunohistochemistry (Frozen sections) - Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (ab244570)](https://www.abcam.com/ps/products/244/ab244570/Images/ab244570-1-ab33945IHCFr1.jpg "Immunohistochemistry (Frozen sections) - Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (ab244570)")
Key features and details
- Mouse monoclonal [MRC OX-22] to CD45RC - BSA and Azide free
- Suitable for: IHC-Fr, IHC-P, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free
See all CD45RC primary antibodies -
Description
Mouse monoclonal [MRC OX-22] to CD45RC - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Rat CD45RC. PHA stimulated rat lymphocytes.
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Positive control
- IHC-Fr: Rat spleen tissue.
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General notes
ab244570 is the carrier-free version of ab33945.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MRC OX-22 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use a concentration of 1 µg/ml.
This product gave a positive signal in HeLa cells fixed with 10% formaldehyde (10 min). |
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-Fr
Use a concentration of 1 µg/ml. This product gave a positive signal in HeLa cells fixed with 10% formaldehyde (10 min). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
Involvement in disease
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Entrez Gene: 24699 Rat
- Omim: 151460 Human
- SwissProt: P08575 Human
- SwissProt: P04157 Rat
- Unigene: 654514 Human
- Unigene: 90166 Rat
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Alternative names
- B220 antibody
- CD45 antibody
- CD45R antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (ab244570)
IHC image of CD45RC staining in a section of frozen normal rat spleen*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab33945 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33945.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from Charles River.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium Azide (ab33945)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab244570 has been referenced in 1 publication.
- Spickett GP et al. MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen. J Exp Med 158:795-810 (1983). PubMed: 6224884
