Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-CD46 antibody [EPR4014] (HRP) (ab196863)

Overview

  • Product name

    Anti-CD46 antibody [EPR4014] (HRP)
    See all CD46 primary antibodies
  • Description

    Rabbit monoclonal [EPR4014] to CD46 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD46 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa whole cell lysate.
  • General notes

    Alternative versions available:
    Anti-CD46 antibody [EPR4014] (ab108307) - Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: 30% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4014
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
  • Tissue specificity

    Expressed by all cells except erythrocytes.
  • Involvement in disease

    Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) [MIM:612922]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations.
  • Sequence similarities

    Contains 4 Sushi (CCP/SCR) domains.
  • Domain

    Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.
  • Post-translational
    modifications

    N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
    Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
    In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by Lck.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
  • Information by UniProt
  • Database links

  • Alternative names

    • AHUS2 antibody
    • Antigen defined by monoclonal antibody TRA 2 10 antibody
    • Antigen identified by monoclonal antibody TRA 2 10 antibody
    • CD46 antibody
    • CD46 antigen antibody
    • CD46 antigen complement regulatory protein antibody
    • CD46 molecule antibody
    • CD46 molecule complement regulatory protein antibody
    • Complement membrane cofactor protein antibody
    • MCP antibody
    • MCP_HUMAN antibody
    • Measles virus receptor antibody
    • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody
    • Membrane cofactor protein antibody
    • MGC26544 antibody
    • MIC10 antibody
    • TLX antibody
    • TRA2.10 antibody
    • Trophoblast leucocyte common antigen antibody
    • Trophoblast leukocyte common antigen antibody
    • Trophoblast lymphocyte cross reactive antigen antibody
    see all

Images

  • All lanes : Anti-NDUFS3 antibody [EPR12782] - C-terminal (Alexa Fluor® 647) (ab196864) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CD46 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa


    Exposure time: 20 minutes


    ab196863 was shown to recognize CD46 in wild-type HAP1 cells as signal was lost at the expected MW in CD46 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. Ab196863 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • Anti-CD46 antibody [EPR4014] (HRP) (ab196863) at 1/1000 dilution + HeLa whole cell lysate (ab150035) at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 44 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab196863 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab196863 has not yet been referenced specifically in any publications.

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