Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-CD46 antibody [EPR4014] - Low endotoxin, Azide free (ab229446)

Overview

  • Product name

    Anti-CD46 antibody [EPR4014] - Low endotoxin, Azide free
    See all CD46 primary antibodies
  • Description

    Rabbit monoclonal [EPR4014] to CD46 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human CD46.

  • Positive control

    • Molt-4, Jurkat, HeLa, and K562 cell lysates; Human kidney and Tonsil tissue
  • General notes

    ab229446 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4014
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
ICC Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
    • Tissue specificity

      Expressed by all cells except erythrocytes.
    • Involvement in disease

      Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) [MIM:612922]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations.
    • Sequence similarities

      Contains 4 Sushi (CCP/SCR) domains.
    • Domain

      Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.
    • Post-translational
      modifications

      N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
      Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
      In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by Lck.
    • Cellular localization

      Cytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
    • Information by UniProt
    • Database links

    • Alternative names

      • AHUS2 antibody
      • Antigen defined by monoclonal antibody TRA 2 10 antibody
      • Antigen identified by monoclonal antibody TRA 2 10 antibody
      • CD46 antibody
      • CD46 antigen antibody
      • CD46 antigen complement regulatory protein antibody
      • CD46 molecule antibody
      • CD46 molecule complement regulatory protein antibody
      • Complement membrane cofactor protein antibody
      • MCP antibody
      • MCP_HUMAN antibody
      • Measles virus receptor antibody
      • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody
      • Membrane cofactor protein antibody
      • MGC26544 antibody
      • MIC10 antibody
      • TLX antibody
      • TRA2.10 antibody
      • Trophoblast leucocyte common antigen antibody
      • Trophoblast leukocyte common antigen antibody
      • Trophoblast lymphocyte cross reactive antigen antibody
      see all

    Images

    • ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded Human kidney tissue

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

    • ab108307 showing positive staining in Normal placenta tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

    • ab108307 showing positive staining in Normal uterus tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

    • ab108307 showing positive staining in Thyroid gland carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

    • ab108307 showing positive staining in Colonic adenocarcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

    • ab108307 showing positive staining in Breast carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

    • ab108307 showing positive staining in Normal breast tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).

    • This IHC data was generated using the same anti-CD46 antibody clone, EPR4014, in a different buffer formulation (cat# ab108307).

      ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded Human tonsil tissue

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • This WB data was generated using the same anti-CD46 antibody clone, EPR4014, in a different buffer formulation (cat# ab108307).

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CD46 knockout HAP1 cell lysate (20 µg) 
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg) 
      Lanes 1 - 4: Merged signal (red and green). Green - ab108307 observed at 50-70 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab108307 was shown to specifically react with  when CD46 knockout samples were used. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. ab108307 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    References

    ab229446 has not yet been referenced specifically in any publications.

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