Product nameAnti-CD47 antibody
See all CD47 primary antibodies
DescriptionRabbit polyclonal to CD47
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: MCF-7 and SK-OV-3 cell lysates; Mouse lung lysate. IHC: human placenta and human lung cancer tissues. ICC/IF: L929 and U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 35 kDa.|
FunctionHas a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
Tissue specificityVery broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
Formalin-fixed, paraffin-embedded human placenta tissue stained for CD47 using ab175388 at 1/200 dilution in immunohistochemical analysis.
Immunofluorescence staining of L929 cells stained for CD47 with ab175388 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
All lanes : Anti-CD47 antibody (ab175388) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : SK-OV-3 (human ovary adenocarcinoma cell line) cell lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 90 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for CD47 using ab175388 at 1/200 dilution in immunohistochemical analysis.
Immunofluorescence staining of U-2 OS cells stained for CD47 with ab175388 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
This product has been referenced in:
- Wang Y et al. Biomimetic Nanotherapies: Red Blood Cell Based Core-Shell Structured Nanocomplexes for Atherosclerosis Management. Adv Sci (Weinh) 6:1900172 (2019). Read more (PubMed: 31380165) »
- Jellvert Å et al. Castration is a prerequisite for the inhibitory effect of metronomic chemotherapy on the growth of experimental castration-resistant prostate cancer. Acta Oncol 57:895-901 (2018). Read more (PubMed: 29299975) »