Key features and details
- Mouse monoclonal [OX-101] to CD47
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Product nameAnti-CD47 antibody [OX-101]
See all CD47 primary antibodies
DescriptionMouse monoclonal [OX-101] to CD47
Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Rat
Tissue, cells or virus corresponding to Rat CD47. Biotinylated rat (AO) thymocytes.
- Flow Cyt: Lewis rat splenocytes. IHC-Fr: Rat Placenta
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
OX-101 (this clone) recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesab33852 was purified from tissue culture supernatant.
Primary antibody notesOX-101 (this clone) recognises the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).
Light chain typekappa
Our Abpromise guarantee covers the use of ab33852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use a concentration of 0.2 µg/ml.|
FunctionHas a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
Tissue specificityVery broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
Lewis rat splenocytes stained with ab33852 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33852) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
IHC image of CD47 staining in a section of frozen normal Rat Placenta.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab33852 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) and ab150080 (Goat Anti-Rabbit IgG H&L (Alexa Fluor®594) (Shown in red) 1/1000) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
ab33852 has been referenced in 2 publications.
- Nevitt C et al. Physiological levels of thrombospondin-1 decrease NO-dependent vasodilation in coronary microvessels from aged rats. Am J Physiol Heart Circ Physiol 310:H1842-50 (2016). PubMed: 27199114
- Vernon-Wilson EF et al. CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1. Eur J Immunol 30:2130-7 (2000). PubMed: 10940903