APC Anti-CD47 antibody [OX-101] (ab256293)
Key features and details
- APC Mouse monoclonal [OX-101] to CD47
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
APC Anti-CD47 antibody [OX-101]
See all CD47 primary antibodies -
Description
APC Mouse monoclonal [OX-101] to CD47 -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD47. Biotinylated rat (AO) thymocytes.
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Positive control
- Flow Cyt: Lewis rat splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX-101 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256293 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/2500.
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Notes |
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Flow Cyt
1/2500. |
Target
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Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. -
Tissue specificity
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 29364 Rat
- SwissProt: P97829 Rat
- Unigene: 7409 Rat
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Alternative names
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
see all
Images
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Lewis rat splenocytes stained with ab256293 (right) or mouse IgG1κ Allophycocyanin isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256293) or mouse IgG1κ Allophycocyanin isotype (1x 106 in 100 µl at 0.2 µg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter. Events were gated on viable lymphocytes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab256293 has not yet been referenced specifically in any publications.