Overview

  • Product name

    Anti-CD47 antibody [OX-101] - BSA and Azide free
    See all CD47 primary antibodies
  • Description

    Mouse monoclonal [OX-101] to CD47 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat CD47. Biotinylated rat (AO) thymocytes.

  • Positive control

    • Flow Cyt: Lewis rat splenocytes.
  • General notes

    ab244567 is a PBS-only buffer format of ab33852. Please refer to ab33852 for recommended dilutions, protocols, and image data.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    OX-101 (this clone) recognises the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    OX-101
  • Isotype

    IgG
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab244567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 0.2 µg/ml.

Target

  • Function

    Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
  • Tissue specificity

    Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen identified by monoclonal antibody 1D8 antibody
    • Antigenic surface determinant protein OA3 antibody
    • CD 47 antibody
    • CD47 antibody
    • CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
    • CD47 antigen antibody
    • CD47 glycoprotein antibody
    • CD47 molecule antibody
    • CD47_HUMAN antibody
    • IAP antibody
    • Integrin Associated Protein antibody
    • Integrin associated signal transducer antibody
    • Integrin-associated protein antibody
    • Leukocyte surface antigen CD47 antibody
    • MER 6 antibody
    • MER6 antibody
    • OA 3 antibody
    • OA3 antibody
    • OTTHUMP00000041152 antibody
    • OTTHUMP00000041153 antibody
    • Protein MER6 antibody
    • Rh related antigen antibody
    • Surface antigen identified by monoclonal antibody 1D8 antibody
    see all

Images

  • This data was generating using the same clone in a different formulation (ab33852).

    Lewis rat splenocytes stained with ab33852 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33852) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.

    The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

     

References

ab244567 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab244567.
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