Recombinant Anti-CD47 antibody [RM1014] (ab284132)
![Western blot - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-484929-CD47WB1.jpg "Western blot - Anti-CD47 antibody [RM1014] (ab284132)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1014] to CD47
- Suitable for: WB, IHC-P, ICC, Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD47 antibody [RM1014]
See all CD47 primary antibodies -
Description
Rabbit recombinant multiclonal [RM1014] to CD47 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- WB: Jurkat, HEK-293T and U-937 whole cell lysates; human ovary cancer tissue lysate. IHC-P: Human spleen, placenta, breast cancer and ovarian carcinoma tissue. ICC: Jurkat cells. Flow cyt: U-937 and Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
RM1014 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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KO cell lines
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab284132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 47-52 kDa (predicted molecular weight: 35 kDa).
|
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| ICC |
1/50.
|
|
| Flow Cyt |
1/500.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 47-52 kDa (predicted molecular weight: 35 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC
1/50. |
|
Flow Cyt
1/500. |
Target
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Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. -
Tissue specificity
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 961 Human
- Omim: 601028 Human
- SwissProt: Q08722 Human
- Unigene: 446414 Human
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Alternative names
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
see all
Images
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Western blot - Anti-CD47 antibody [RM1014] (ab284132)All lanes : Anti-CD47 antibody [RM1014] (ab284132) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 20ug
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 20ug
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 20ug
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 42-52 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking and diluting buffer and concentration was 5% NFDM/TBST.
Low expression control: HepG2 (PMID: 25721088, 30415063).
We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.
The molecular mass observed is consistent with the literature (PMID 12393467, PMID 11034562).
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![Western blot - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-484931-CD47WB2.jpg)
Western blot - Anti-CD47 antibody [RM1014] (ab284132)All lanes : Anti-CD47 antibody [RM1014] (ab284132) at 1/1000 dilution
Lane 1 : Human liver lysate
Lane 2 : Human ovary cancer lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 47-52 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking and diluting buffer and concentration was 5% NFDM/TBST.
We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.
-
![Western blot - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-484933-CD47KO293T.jpg)
Western blot - Anti-CD47 antibody [RM1014] (ab284132)All lanes : Anti-CD47 antibody [RM1014] (ab284132) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human embryonic kidney epithelial cell, ab255449) whole cell lysate
Lane 2 : CD47 knockout HEK-293T (ab266324) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 47-52 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking and diluting buffer and concentration was 5% NFDM/TBST.
We recommend that samples are not boiled after adding loading buffer as this may cause protein aggregates.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-2-anti-cd47-antibody-oligo14-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)Immunohistochemical analysis of paraffin-embedded human spleen tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human spleen. The section was incubated with ab284132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-3-anti-cd47-antibody-oligo14-immunohistochemistry-human-placenta.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)Immunohistochemical analysis of paraffin-embedded human placenta tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on human placenta. The section was incubated with ab284132 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-4-anti-cd47-antibody-oligo14-immunohistochemistry-human-breast-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™Polymer Refine Detection). Positive staining on human breast cancer (PMID: 31528120). The section was incubated with ab284132 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-5-anti-cd47-antibody-oligo14-immunohistochemistry-human-ovarian-carcin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labelling CD47 with ab284132 at 1/500 (0.924 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used. Positive staining on human ovarian carcinoma. The section was incubated with ab284132 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-6-anti-cd47-antibody-oligo14-immunohistochemistry-human-ovarian-carcin1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [RM1014] (ab284132)Immunohistochemical analysis of paraffin-embedded human ovarian carcin tissue labelling CD47 with ab284132 at 1/500 followed by a ready to use LeicaDS9800 (BOND™Polymer Refine Detection) was used. Positive staining on human ovarian carcinoma. The section was incubated with ab284132 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunocytochemistry - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-7-anti-cd47-antibody-oligo14-immunocytochemistry-jurkat.jpg)
Immunocytochemistry - Anti-CD47 antibody [RM1014] (ab284132)Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat cells labelling CD47 with ab284132 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in Jurkat cell line. Negative control: HepG2 (PMID: 25721088). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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![Flow Cytometry - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-8-anti-cd47-antibody-oligo14-flow-cytometry-hepg2.jpg)
Flow Cytometry - Anti-CD47 antibody [RM1014] (ab284132)Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell, Left) / U-937 (Human histiocytic lymphoma monocyte, Right) cells labelling CD47 with ab284132 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Low expression control: HepG2 (PMID: 25721088, 30415063). Gated on viable cells.
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![Flow Cytometry - Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-9-anti-cd47-antibody-oligo14-flow-cytometry-hepg21.jpg)
Flow Cytometry - Anti-CD47 antibody [RM1014] (ab284132)Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell, Left) / Jurkat (Human T cell leukemia T lymphocyte, Right) cells labelling CD47 with ab284132 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Low expression control: HepG2 (PMID: 25721088, 30415063). Gated on viable cells.
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![Anti-CD47 antibody [RM1014] (ab284132)](https://www.abcam.com/ps/products/284/ab284132/Images/ab284132-484859-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD47 antibody [RM1014] (ab284132)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284132 has not yet been referenced specifically in any publications.