Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP279] to CD47
- Suitable for: IHC-P
- Reacts with: Human
Product nameAnti-CD47 antibody [SP279]
See all CD47 primary antibodies
DescriptionRabbit monoclonal [SP279] to CD47
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD47 aa 50-150 (internal sequence). The exact sequence is proprietary.
Database link: Q08722
- IHC-P: Human ovarian adenocarcinoma and prostate adenocarcinoma tissues.
FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab226837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionHas a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
Tissue specificityVery broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane.
- Information by UniProt
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD47 using ab226837 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for CD47 using ab226837 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226837 has been referenced in 1 publication.
- Papalampros A et al. Unique Spatial Immune Profiling in Pancreatic Ductal Adenocarcinoma with Enrichment of Exhausted and Senescent T Cells and Diffused CD47-SIRPa Expression. Cancers (Basel) 12:N/A (2020). PubMed: 32645996