RecombinantRabMAb

Recombinant Anti-CD47 antibody [SP279] - BSA and Azide free (ab236234)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP279] to CD47 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD47 antibody [SP279] - BSA and Azide free
    See all CD47 primary antibodies

  • Description

    Rabbit monoclonal [SP279] to CD47 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD47 aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: Q08722

  • Positive control

    • IHC-P: Human prostate adenocarcinoma tissue.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab236234 is the carrier-free version of ab226837. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab236234 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.20
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP279

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

  • Tissue specificity

    Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen identified by monoclonal antibody 1D8 antibody
      • Antigenic surface determinant protein OA3 antibody
      • CD 47 antibody
      • CD47 antibody
      • CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
      • CD47 antigen antibody
      • CD47 glycoprotein antibody
      • CD47 molecule antibody
      • CD47_HUMAN antibody
      • IAP antibody
      • Integrin Associated Protein antibody
      • Integrin associated signal transducer antibody
      • Integrin-associated protein antibody
      • Leukocyte surface antigen CD47 antibody
      • MER 6 antibody
      • MER6 antibody
      • OA 3 antibody
      • OA3 antibody
      • OTTHUMP00000041152 antibody
      • OTTHUMP00000041153 antibody
      • Protein MER6 antibody
      • Rh related antigen antibody
      • Surface antigen identified by monoclonal antibody 1D8 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [SP279] - BSA and Azide free (ab236234)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [SP279] - BSA and Azide free (ab236234)

      Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for CD47 using ab226837 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226837).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [SP279] - BSA and Azide free (ab236234)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [SP279] - BSA and Azide free (ab236234)

      Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD47 using ab226837 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab226837).

       

    • Anti-CD47 antibody [SP279] - BSA and Azide free (ab236234)
      Anti-CD47 antibody [SP279] - BSA and Azide free (ab236234)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab236234? Please let us know so that we can cite the reference in this datasheet.

    ab236234 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com