Anti-CD5 antibody (ab232713)
Key features and details
- Rabbit polyclonal to CD5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD5 antibody
See all CD5 primary antibodies -
Description
Rabbit polyclonal to CD5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD5 aa 1-400. N-terminal His-tag and GST-tag; expressed in E.coli.
Database link: P06127 -
Positive control
- WB: A549 and HepG2 cell lysate; Recombinant human CD5 protein. IHC-P: Human liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 55 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 55 kDa. |
Target
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Function
May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2. -
Sequence similarities
Contains 3 SRCR domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 921 Human
- Omim: 153340 Human
- SwissProt: P06127 Human
- Unigene: 58685 Human
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Alternative names
- CD 5 antibody
- CD5 antibody
- CD5 antigen (p56 62) antibody
see all
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for CD5 using ab232713 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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Anti-CD5 antibody (ab232713) at 1 µg/ml + Recombinant human CD5 protein
Predicted band size: 55 kDa -
Anti-CD5 antibody (ab232713) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 55 kDa -
Anti-CD5 antibody (ab232713) at 1 µg/ml + A549 (human lung carcinoma cell line) cell lysate
Predicted band size: 55 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232713 has not yet been referenced specifically in any publications.