Anti-CD5 antibody [C5/473 + CD5/54/F6] (ab200697)

Mouse monoclonal CD5 antibody [C5/473 + CD5/54/F6]. Validated in IP, IHC, Flow Cyt, ICC/IF and tested in Rat, Rabbit, Cow, Human. Immunogen corresponding to recombinant fragment.

Overview

  • Product name

    Anti-CD5 antibody [C5/473 + CD5/54/F6]
    See all CD5 primary antibodies
  • Description

    Mouse monoclonal [C5/473 + CD5/54/F6] to CD5
  • Host species

    Mouse
  • Specificity

    ab200697 does not react with granulocytes or monocytes.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Rat, Rabbit, Cow, Human
  • Immunogen

    Recombinant fragment corresponding to Human CD5. Clone C5/473
    Database link: P06127

  • Positive control

    • 293T, Ramos, or MOLT4 cells; Human tonsil tissue.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

    Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-).

Properties

Applications

Our Abpromise guarantee covers the use of ab200697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

for 30 minutes at RT

IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

for 30 minutes at RT.

IP Use 0.5-1 µg for 500 µg of chromatin.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD5 with ab200697 at 1 µg/ml.

References

ab200697 has not yet been referenced specifically in any publications.

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