Recombinant
RabMAb

Recombinant Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

Overview

  • Product name

    Anti-CD5 antibody [EP2952] - BSA and Azide free
    See all CD5 primary antibodies
  • Description

    Rabbit monoclonal [EP2952] to CD5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD5 aa 50-150. The exact sequence is proprietary.

  • General notes

    ab194821 is the carrier-free version of ab75877 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab194821 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab194821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.

WB Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
    • Sequence similarities

      Contains 3 SRCR domains.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 5 antibody
      • CD5 antibody
      • CD5 antigen (p56 62) antibody
      • CD5 antigen antibody
      • CD5 molecule antibody
      • CD5_HUMAN antibody
      • LEU1 antibody
      • LyA antibody
      • Lymphocyte antigen T1/Leu-1 antibody
      • OTTHUMP00000236973 antibody
      • T-cell surface glycoprotein CD5 antibody
      • T1 antibody
      see all

    Images

    • ab75877 showing negative staining in Normal brain tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab75877 showing positive staining in Normal colon lymphocytes tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab75877 showing negative staining in Normal kidney tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab75877 showing negative staining in Skeletal muscle tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab75877 showing positive staining in Normal spleen tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

    References

    ab194821 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab194821.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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