The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use neat for 10 minutes at room temperature. Antigen retrieval is not essential however staining may be enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.