Recombinant

Recombinant Anti-CD52 antibody [Campath-1H] (ab245681)

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Overview

  • Product name

    Anti-CD52 antibody [Campath-1H]
    See all CD52 primary antibodies

  • Description

    Rabbit monoclonal [Campath-1H] to CD52

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey

  • Immunogen

    Tissue, cells or virus corresponding to CD52. Human lymphocytes.

  • Positive control

    • IHC-P: Rat spleen, thymus and epididymis tissue. Human thyroid cancer tissue. Flow Cyt: Rhesus and cynomolgus monkey lymphocytes.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Proclin
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    Campath-1H

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)

    Formalin-fixed human thyroid cancer tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunocytochemistry/ Immunofluorescence - Anti-CD52 antibody [Campath-1H] (ab245681)

    Rat thymus tissue stained for CD52 with ab245681 at 5 µg/ml followed by an FITC conjugated secondary antibody in immunohistochemical analysis. The nuclear stain is DAPI (blue). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)

    Formalin-fixed, paraffin-embedded rat thymus tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)

    Formalin-fixed, paraffin-embedded rat spleen tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)

    Formalin-fixed, paraffin-embedded rat epididymis tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.

  • Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)
    Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)

    Flow-cytometry using ab245681. 

    Cynomolgus monkey lymphocytes were stained with an isotype control (Panel A) or ab245681 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).

  • Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)
    Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)

    Flow-cytometry using ab245681. 

    Rhesus monkey lymphocytes were stained with an isotype control (Panel A) or ab245681 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 ® conjugated donkey anti-rabbit antibody (ab150073).

References

ab245681 has not yet been referenced specifically in any publications.

Publishing research using ab245681? Please let us know so that we can cite the reference in this datasheet.

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