Recombinant Anti-CD52 antibody [Campath-1H] (ab245681)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Campath-1H] to CD52
- Suitable for: IHC-P, Flow Cyt, ICC/IF
- Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey
Overview
-
Product name
Anti-CD52 antibody [Campath-1H]
See all CD52 primary antibodies -
Description
Rabbit monoclonal [Campath-1H] to CD52 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey -
Immunogen
Tissue, cells or virus corresponding to CD52. Human lymphocytes.
-
Positive control
- IHC-P: Rat spleen, thymus and epididymis tissue. Human thyroid cancer tissue. Flow Cyt: Rhesus and cynomolgus monkey lymphocytes.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Campath-1H -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab245681 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | Use a concentration of 5 µg/ml. | |
Flow Cyt | Use a concentration of 1 µg/ml. | |
ICC/IF | Use a concentration of 5 µg/ml. |
Target
-
Function
May play a role in carrying and orienting carbohydrate, as well as having a more specific role. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1043 Human
- Entrez Gene: 117054 Rat
- Entrez Gene: 714429 Rhesus monkey
- Omim: 114280 Human
- SwissProt: P31358 Human
- SwissProt: Q63064 Rat
- Unigene: 276770 Human
- Unigene: 34155 Rat
-
Alternative names
- Cambridge pathology 1 antigen antibody
- CAMPATH 1 antibody
- CAMPATH 1 antigen antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
Formalin-fixed human thyroid cancer tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.
-
Rat thymus tissue stained for CD52 with ab245681 at 5 µg/ml followed by an FITC conjugated secondary antibody in immunohistochemical analysis. The nuclear stain is DAPI (blue).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
Formalin-fixed, paraffin-embedded rat thymus tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
Formalin-fixed, paraffin-embedded rat spleen tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
Formalin-fixed, paraffin-embedded rat epididymis tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.
-
Flow-cytometry using ab245681.
Cynomolgus monkey lymphocytes were stained with an isotype control (Panel A) or ab245681 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).
-
Flow-cytometry using ab245681.
Rhesus monkey lymphocytes were stained with an isotype control (Panel A) or ab245681 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 ® conjugated donkey anti-rabbit antibody (ab150073).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab245681 has not yet been referenced specifically in any publications.