Recombinant

Recombinant Anti-CD52 antibody [Campath-1H] (ab245681)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD52 antibody [Campath-1H] (ab245681)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
  • Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)
  • Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)
  • Anti-CD52 antibody [Campath-1H] (ab245681)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Campath-1H] to CD52
  • Suitable for: IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey

Overview

  • Product name

    Anti-CD52 antibody [Campath-1H]
    See all CD52 primary antibodies

  • Description

    Rabbit monoclonal [Campath-1H] to CD52

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human, Cynomolgus monkey, Rhesus monkey

  • Immunogen

    Tissue, cells or virus corresponding to CD52. Human lymphocytes.

  • Positive control

    • IHC-P: Rat spleen, thymus and epididymis tissue. Human thyroid cancer tissue. Flow Cyt: Rhesus and cynomolgus monkey lymphocytes.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Proclin 300
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    Campath-1H

  • Isotype

    IgG

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)

    Formalin-fixed human thyroid cancer tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunocytochemistry/ Immunofluorescence - Anti-CD52 antibody [Campath-1H] (ab245681)

    Rat thymus tissue stained for CD52 with ab245681 at 5 µg/ml followed by an FITC conjugated secondary antibody in immunohistochemical analysis. The nuclear stain is DAPI (blue). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)

    Formalin-fixed, paraffin-embedded rat thymus tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)

    Formalin-fixed, paraffin-embedded rat spleen tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD52 antibody [Campath-1H] (ab245681)

    Formalin-fixed, paraffin-embedded rat epididymis tissue stained for CD52 with ab245681 at 5 µg/ml in immunohistochemical analysis.

  • Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)
    Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)

    Flow-cytometry using ab245681. 

    Cynomolgus monkey lymphocytes were stained with an isotype control (Panel A) or ab245681 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 conjugated donkey anti-rabbit antibody (ab150073).

  • Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)
    Flow Cytometry - Anti-CD52 antibody [Campath-1H] (ab245681)

    Flow-cytometry using ab245681. 

    Rhesus monkey lymphocytes were stained with an isotype control (Panel A) or ab245681 (Panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an AF488 ® conjugated donkey anti-rabbit antibody (ab150073).

  • Anti-CD52 antibody [Campath-1H] (ab245681)
    Anti-CD52 antibody [Campath-1H] (ab245681)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab245681? Please let us know so that we can cite the reference in this datasheet.

ab245681 has not yet been referenced specifically in any publications.

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