Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23855-41] to CD52 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Product nameAnti-CD52 antibody [EPR23855-41] - BSA and Azide free
See all CD52 primary antibodies
DescriptionRabbit monoclonal [EPR23855-41] to CD52 - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD52 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P31358
- Flow cyt: Human PBMCs.
ab275257 is the carrier-free version of ab259794. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab275257 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab275257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionMay play a role in carrying and orienting carbohydrate, as well as having a more specific role.
Cellular localizationCell membrane.
- Information by UniProt
- Cambridge pathology 1 antigen antibody
- CAMPATH 1 antibody
- CAMPATH 1 antigen antibody
This data was developed using ab259794, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD52 with ab259794 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control.
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab259794. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.
Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab275257 has not yet been referenced specifically in any publications.