Overview

  • Product name

    Anti-CD52 antibody [YTH34.5] - Low endotoxin, Azide free
    See all CD52 primary antibodies
  • Description

    Rat monoclonal [YTH34.5] to CD52 - Low endotoxin, Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: Functional Studies, WB, IP, IHC-P, IHC-Fr, Flow Cyt, IHC-Rmore details
  • Species reactivity

    Reacts with: Human, Rhesus monkey
  • Immunogen

    Tissue, cells or virus corresponding to Human CD52. (Human lymphocytes).

  • Positive control

    • Human peripheral blood lymphocytes; Human tonsil tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    ab194860 was prepared by affinity chromatography on Protein A from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    YTH34.5
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use a concentration of 50 µg/ml.

Cytotoxic Assays (Use human serum as complement source).

WB Use at an assay dependent concentration. Predicted molecular weight: 6 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100.

Use 10 µl of the suggested working dilution to label 1 x 106 cells in 100 µl.

 

 

 

 

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

IHC-R Use at an assay dependent concentration.

Target

  • Function

    May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cambridge pathology 1 antigen antibody
    • CAMPATH 1 antibody
    • CAMPATH 1 antigen antibody
    • CAMPATH-1 antigen antibody
    • CD 52 antibody
    • CD52 antibody
    • CD52 antigen antibody
    • CD52 molecule antibody
    • CD52_HUMAN antibody
    • CDw52 antibody
    • CDW52 antigen antibody
    • Epididymal secretory protein E5 antibody
    • Epididymis secretory sperm binding protein Li 171mP antibody
    • HE 5 antibody
    • He5 antibody
    • Human epididymis-specific protein 5 antibody
    see all

Images

  • Flow cytometric analysis of Human peripheral blood lymphocytes labeling CD52 with ab194860 at 1/50 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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