Recombinant
RabMAb

Recombinant Anti-CD53 antibody [EPR4342(2)] (ab134094)

Overview

  • Product name

    Anti-CD53 antibody [EPR4342(2)]
    See all CD53 primary antibodies
  • Description

    Rabbit monoclonal [EPR4342(2)] to CD53
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus in Human CD53 (UniProt P19397).

  • Positive control

    • Raji, PBMC, and Jurkat whole cell lysate (ab7899); Human tonsil tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4342(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
IHC-P 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May be involved in growth regulation in hematopoietic cells.
    • Tissue specificity

      B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • AI323659 antibody
      • Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
      • CD 53 antibody
      • CD53 antibody
      • CD53 antigen antibody
      • CD53 antigen, isoform CRA_b antibody
      • CD53 glycoprotein antibody
      • Cd53 molecule antibody
      • CD53 tetraspan antigen antibody
      • CD53_HUMAN antibody
      • Cell surface glycoprotein CD53 antibody
      • Leukocyte antigen MRC OX-44 antibody
      • Leukocyte surface antigen CD53 antibody
      • MOX 44 antibody
      • MOX44 antibody
      • OTTHUMP00000059505 antibody
      • Ox-44 antibody
      • OX44 antibody
      • RATOX44 antibody
      • Tetraspanin 25 antibody
      • Tetraspanin-25 antibody
      • Tetraspanin25 antibody
      • Transmembrane glycoprotein antibody
      • TSPAN 25 antibody
      • Tspan-25 antibody
      • TSPAN25 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CD53 with ab134094 at 1/100 dilution.
    • All lanes : Anti-CD53 antibody [EPR4342(2)] (ab134094) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : PBMC cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 24 kDa

    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD53 with unpurified ab134094 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    References

    ab134094 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134094.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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