Key features and details
- Mouse monoclonal [MEM-53] to CD53 (FITC)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-CD53 antibody [MEM-53] (FITC)
See all CD53 primary antibodies
DescriptionMouse monoclonal [MEM-53] to CD53 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Specificityab68565 reacts with CD53 exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Leukocytes of patient suffering from LGL-type leukemia.
- Human blood cells.
The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab68565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMay be involved in growth regulation in hematopoietic cells.
Tissue specificityB-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
Overlay histogram showing peripheral blood lymphocytes stained with ab68565 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68565, 1 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
ab68565 has been referenced in 2 publications.
- Rasmussen AM et al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 153:4997-5007 (1994). PubMed: 7963560
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480