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Immunology Cell Type Markers CD Non-lineage
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Anti-CD53 antibody [MRC OX-44] (ab22383)

  • Datasheet
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Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)

    Key features and details

    • Mouse monoclonal [MRC OX-44] to CD53
    • Suitable for: Flow Cyt
    • Reacts with: Rat
    • Isotype: IgG1

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-CD53 antibody [MRC OX-44]
      See all CD53 primary antibodies
    • Description

      Mouse monoclonal [MRC OX-44] to CD53
    • Host species

      Mouse
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Rat
    • Immunogen

      Tissue, cells or virus corresponding to Rat CD53. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen

    • Positive control

      • Flow Cyt: Lewis rat splenocytes.
    • General notes

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified from TCS.
    • Primary antibody notes

      The antibody recognises the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labelled dendritic cells, granulocytes and microglial cells in the brain.
    • Clonality

      Monoclonal
    • Clone number

      MRC OX-44
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Signal Transduction
      • Adapters
      • Transmembrane

    Associated products

    • Alternative Versions

      • Anti-CD53 antibody [MRC OX-44] - BSA and Azide free (ab244561)
      • Alexa Fluor® 488 Anti-CD53 antibody [MRC OX-44] (ab256285)
      • APC Anti-CD53 antibody [MRC OX-44] (ab256288)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Related Products

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Anti-CD53 antibody [MRC OX-44] - BSA and Azide free (ab244561)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab22383 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use a concentration of 0.2 µg/ml.
    Notes
    Flow Cyt
    Use a concentration of 0.2 µg/ml.

    Target

    • Function

      May be involved in growth regulation in hematopoietic cells.
    • Tissue specificity

      B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P19397 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 24251 Rat
      • SwissProt: P24485 Rat
      • Unigene: 31988 Rat
      • Alternative names

        • AI323659 antibody
        • Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
        • CD 53 antibody
        • CD53 antibody
        • CD53 antigen antibody
        • CD53 antigen, isoform CRA_b antibody
        • CD53 glycoprotein antibody
        • Cd53 molecule antibody
        • CD53 tetraspan antigen antibody
        • CD53_HUMAN antibody
        • Cell surface glycoprotein CD53 antibody
        • Leukocyte antigen MRC OX-44 antibody
        • Leukocyte surface antigen CD53 antibody
        • MOX 44 antibody
        • MOX44 antibody
        • OTTHUMP00000059505 antibody
        • Ox-44 antibody
        • OX44 antibody
        • RATOX44 antibody
        • Tetraspanin 25 antibody
        • Tetraspanin-25 antibody
        • Tetraspanin25 antibody
        • Transmembrane glycoprotein antibody
        • TSPAN 25 antibody
        • Tspan-25 antibody
        • TSPAN25 antibody
        see all

      Images

      • Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)
        Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)

        Lewis rat splenocytes stained with ab22383 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22383) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.

        The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

        Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

         

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab22383? Please let us know so that we can cite the reference in this datasheet.

      ab22383 has been referenced in 2 publications.

      • Tomlinson MG  et al. Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the Transmembrane 4 Superfamily. Eur J Immunol 23:136-40 (1993). PubMed: 7678222
      • Paterson DJ  et al. The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes. J Exp Med 165:1-13 (1987). PubMed: 3098892

      Customer reviews and Q&As

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