Anti-CD53 antibody [MRC OX-44] (ab22383)
![Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)](https://www.abcam.com/ps/products/22/ab22383/Images/ab22383-1-ab22383Flow1.jpg "Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)")
Key features and details
- Mouse monoclonal [MRC OX-44] to CD53
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD53 antibody [MRC OX-44]
See all CD53 primary antibodies -
Description
Mouse monoclonal [MRC OX-44] to CD53 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD53. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen
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Positive control
- Flow Cyt: Lewis rat splenocytes.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Primary antibody notes
The antibody recognises the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labelled dendritic cells, granulocytes and microglial cells in the brain. -
Clonality
Monoclonal -
Clone number
MRC OX-44 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 0.2 µg/ml.
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| Notes |
|---|
|
Flow Cyt
Use a concentration of 0.2 µg/ml. |
Target
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Function
May be involved in growth regulation in hematopoietic cells. -
Tissue specificity
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 24251 Rat
- SwissProt: P24485 Rat
- Unigene: 31988 Rat
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Alternative names
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
see all
Images
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Flow Cytometry - Anti-CD53 antibody [MRC OX-44] (ab22383)
Lewis rat splenocytes stained with ab22383 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22383) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab22383 has been referenced in 2 publications.
- Tomlinson MG et al. Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the Transmembrane 4 Superfamily. Eur J Immunol 23:136-40 (1993). PubMed: 7678222
- Paterson DJ et al. The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes. J Exp Med 165:1-13 (1987). PubMed: 3098892