Key features and details
- Mouse monoclonal [MRC OX-44] to CD53 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-CD53 antibody [MRC OX-44] - BSA and Azide free
See all CD53 primary antibodies
DescriptionMouse monoclonal [MRC OX-44] to CD53 - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Rat
Tissue, cells or virus corresponding to Rat CD53. T lymphocytes from a mixed lymphocyte reaction between purified rat T helper cells and irradiated semiallogeneic rat spleen
- Flow Cyt: Lewis rat splenocytes.
ab244561 is the PBS only version of ab22383.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact firstname.lastname@example.org.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Clone numberMRC OX-44
Light chain typekappa
Our Abpromise guarantee covers the use of ab244561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 0.2 µg/ml.|
FunctionMay be involved in growth regulation in hematopoietic cells.
Tissue specificityB-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
This data was generating using the same clone in a different formulation (ab22383).
Lewis rat splenocytes stained with ab22383 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22383) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244561 has not yet been referenced specifically in any publications.