Anti-CD55 antibody [67] (ab20145)
Key features and details
- Mouse monoclonal [67] to CD55
- Suitable for: IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD55 antibody [67]
See all CD55 primary antibodies -
Description
Mouse monoclonal [67] to CD55 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD55. Fibronectin purified monocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
67 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab20145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
Fix with acetone. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. Fix with acetone. |
Target
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Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. -
Tissue specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains. -
Domain
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3. -
Post-translational
modificationsThe Ser/Thr-rich domain is heavily O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1604 Human
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
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Alternative names
- CD 55 antibody
- CD55 antibody
- CD55 antigen antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab20145 has been referenced in 6 publications.
- Bahia El Idrissi N et al. Complement activation at the motor end-plates in amyotrophic lateral sclerosis. J Neuroinflammation 13:72 (2016). PubMed: 27056040
- Natsume A et al. Engineered anti-CD20 antibodies with enhanced complement-activating capacity mediate potent anti-lymphoma activity. Cancer Sci 100:2411-8 (2009). PubMed: 19758394
- Kamada K et al. Expression of sphingosine kinase 2 in synovial fibroblasts of rheumatoid arthritis contributing to apoptosis by a sphingosine analogue, FTY720. Pathol Int 59:382-9 (2009). IHC-Fr ; Human . PubMed: 19490468
- Franchi A et al. Expression of immunomodulatory genes, their protein products and specific ligands/receptors during the window of implantation in the human endometrium. Mol Hum Reprod 14:413-21 (2008). PubMed: 18524812
- Leitinger B & Hogg N The involvement of lipid rafts in the regulation of integrin function. J Cell Sci 115:963-72 (2002). PubMed: 11870215
- Palmer DG et al. Features of synovial membrane identified with monoclonal antibodies. Clin Exp Immunol 59:529-38 (1985). PubMed: 3886218