Immunohistochemistry (Frozen sections) abreview for Anti-CD55 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Adult Brain, 40 micron, floating)
Specification
Adult Brain, 40 micron, floating
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Diluent: 5% Donkey Serum, PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Chicken, 546 (Molecular Probes)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/1000

Additional data

Additional Notes
Tried at various concentrations (1:100-1:2500) with and without .25% triton. (1:100 shown) Labels neurons in the central nervous system, with intensity dependent on concentration. Triton had little impact. Since there is limited literature about the expression of CD55 in the CNS, it is unclear if this labeling is accurate or not, thus the inconclusive rating.
Abcam response
Thank you for your honest feedback regarding this antibody.

As the antibody had not previously been characterized in IHC or mouse samples, I would recommend a positive control (one possibility is human lung) to validate the IHC staining procedure.

Dr. Joseph Dougherty

Verified customer

Submitted Aug 10 2007

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