Recombinant
RabMAb

Recombinant Anti-CD55 antibody [EPR22362-255] (ab243231)

Overview

  • Product name

    Anti-CD55 antibody [EPR22362-255]
    See all CD55 primary antibodies
  • Description

    Rabbit monoclonal [EPR22362-255] to CD55
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD55 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P08174

  • Positive control

    • ICC/IF: Human peripheral blood mononuclear cells. Flow cyt: Human peripheral blood mononuclear cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22362-255
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab243231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/500.
  • Application notes
    Is unsuitable for IHC-P or WB.
  • Target

    • Function

      This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
    • Tissue specificity

      Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
    • Sequence similarities

      Belongs to the receptors of complement activation (RCA) family.
      Contains 4 Sushi (CCP/SCR) domains.
    • Domain

      The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
    • Post-translational
      modifications

      The Ser/Thr-rich domain is heavily O-glycosylated.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 55 antibody
      • CD55 antibody
      • CD55 antigen antibody
      • CD55 Cromer blood group system antibody
      • CD55 molecule (Cromer blood group) antibody
      • CD55 molecule antibody
      • CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
      • Cd55a antibody
      • Complement decay accelerating factor antibody
      • Complement decay-accelerating factor antibody
      • Complement decay-accelerating factor, GPI-anchored antibody
      • CR antibody
      • CROM antibody
      • Cromer Blood Group antigen antibody
      • Cromer blood group system antibody
      • DAF antibody
      • Daf-GPI antibody
      • DAF_HUMAN antibody
      • Daf1 antibody
      • Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody
      • Decay accelarating factor 1, isoform CRA_a antibody
      • Decay accelerating factor (GPI-form) antibody
      • Decay Accelerating Factor for Complement antibody
      • Decay accelerating factor GPI-form antibody
      • Decay accelerating factor soluble-form antibody
      • GPI-DAF antibody
      • TC antibody
      see all

    Images

    • Flow cytometric analysis of IMR-32 (human neuroblastoma neuroblast cell line) cells  labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ® 488), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

      Negative control: IMR-32 (PMID: 27043658 and 15699172).

    • Flow cytometric analysis of human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ® 488), at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

      Relative levels of CD55 expression on monocytes (SSC high population) and lymphocyte (main population, SSC low) subsets was described in the literature (PMID: 16999828).

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in human PBMC cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution. 

      Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Negative control: IMR-32 (PMID: 27043658).

    References

    ab243231 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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