Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22362-255] to CD55
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-CD55 antibody [EPR22362-255]
See all CD55 primary antibodies
DescriptionRabbit monoclonal [EPR22362-255] to CD55
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or WB
Species reactivityReacts with: Human
Recombinant fragment within Human CD55 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P08174
- ICC/IF: Human peripheral blood mononuclear cells. Flow cyt: Human peripheral blood mononuclear cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab243231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionThis protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
Tissue specificityExpressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Sequence similaritiesBelongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains.
DomainThe first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
modificationsThe Ser/Thr-rich domain is heavily O-glycosylated.
Cellular localizationCell membrane.
- Information by UniProt
- CD 55 antibody
- CD55 antibody
- CD55 antigen antibody
Flow cytometric analysis of IMR-32 (human neuroblastoma neuroblast cell line) cells labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ® 488), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: IMR-32 (PMID: 27043658 and 15699172).
Flow cytometric analysis of human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/500 (Right panel) compared with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Left panel). Goat anti rabbit IgG (Dylight ® 488), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Relative levels of CD55 expression on monocytes (SSC high population) and lymphocyte (main population, SSC low) subsets was described in the literature (PMID: 16999828).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human peripheral blood mononuclear cells labeling CD55 with ab243231 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing Membranous staining in human PBMC cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.
Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
Negative control: IMR-32 (PMID: 27043658).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243231 has not yet been referenced specifically in any publications.