Recombinant Anti-CD55 antibody [EPR6689] (ab133684)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6689] to CD55
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD55 antibody [EPR6689]
See all CD55 primary antibodies -
Description
Rabbit monoclonal [EPR6689] to CD55 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, and HeLa lysates. IHC-P: human cervix carcinoma, lung and placenta tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.25 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6689 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 41 kDa.
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IHC-P | (1) |
1/4000 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Foe unpurified use at 1/100 - 1/250 |
Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 41 kDa. |
IHC-P
1/4000 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Foe unpurified use at 1/100 - 1/250 |
Target
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Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. -
Tissue specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains. -
Domain
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3. -
Post-translational
modificationsThe Ser/Thr-rich domain is heavily O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1604 Human
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
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Alternative names
- CD 55 antibody
- CD55 antibody
- CD55 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue sections labeling CD55 with purified ab133684 at 1/4000 dilution (0.04 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-CD55 antibody [EPR6689] (ab133684) at 1/15000 dilution (Purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted?CD55 is a 70- to 75-kDa GPI-anchored membrane protein (PMID: 8764022)
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All lanes : Anti-CD55 antibody [EPR6689] (ab133684) at 1/10000 dilution ((unpurified))
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa -
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling CD55 with unpurified ab133684 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling CD55 with unpurified ab133684 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab133684 has been referenced in 11 publications.
- Chen Y et al. Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis. Theranostics 12:1074-1096 (2022). PubMed: 35154475
- Tang Z et al. CD55 is upregulated by cAMP/PKA/AKT and modulates human decidualization via Src and ERK pathway and decidualization-related genes. Mol Reprod Dev 89:256-268 (2022). PubMed: 35474595
- Santos NB et al. Complement Decay-Accelerating Factor is a modulator of influenza A virus lung immunopathology. PLoS Pathog 17:e1009381 (2021). PubMed: 34197564
- Shin DI et al. Motorized Shaver Harvest Results in Similar Cell Yield and Characteristics Compared With Rongeur Biopsy During Arthroscopic Synovium-Derived Mesenchymal Stem Cell Harvest. Arthroscopy 37:2873-2882 (2021). PubMed: 33798652
- Pan J et al. Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution. Front Cell Dev Biol 9:758339 (2021). PubMed: 34805166