FITC Anti-CD55 antibody [MEM-118], prediluted (ab28112)
Key features and details
- FITC Mouse monoclonal [MEM-118] to CD55, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgM
Related conjugates and formulations
Overview
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Product name
FITC Anti-CD55 antibody [MEM-118], prediluted
See all CD55 primary antibodies -
Description
FITC Mouse monoclonal [MEM-118] to CD55, prediluted -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab28112 recognizes an epitope in SCR4 domain of CD55 antigen (Decay accelerating factor). -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates -
Immunogen
Tissue, cells or virus corresponding to Human CD55. T cell line HPB-ALL
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General notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 8.0
Preservative: 0.097% Sodium azide
Constituents: Tris buffered saline, 0.2% BSA -
Concentration information loading...
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Purity
Purified IgM -
Clonality
Monoclonal -
Clone number
MEM-118 -
Isotype
IgM -
Research areas
Associated products
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Alternative Versions
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 10µl for 106 cells.
ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 10µl for 106 cells. ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
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Target
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Function
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. -
Tissue specificity
Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. -
Sequence similarities
Belongs to the receptors of complement activation (RCA) family.
Contains 4 Sushi (CCP/SCR) domains. -
Domain
The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3. -
Post-translational
modificationsThe Ser/Thr-rich domain is heavily O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1604 Human
- Omim: 125240 Human
- SwissProt: P08174 Human
- Unigene: 126517 Human
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Alternative names
- CD 55 antibody
- CD55 antibody
- CD55 antigen antibody
see all
Images
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Overlay histogram showing THP1 cells stained with ab28112 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28112, 10µl/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM (FITC) (ab91546, 10µl/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab28112 has not yet been referenced specifically in any publications.