Overview

  • Product name
    Anti-CD57 antibody [HNK-1/ Leu-7]
    See all CD57 primary antibodies
  • Description
    Mouse monoclonal [HNK-1/ Leu-7] to CD57
  • Host species
    Mouse
  • Specificity
    ab187274 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. ab187274 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. ab187274 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Human CD57. Human peripheral blood mononuclear cells.
    Database link: Q9P2W7

  • Positive control
    • Lymph node, Tonsil

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    HNK-1/ Leu-7
  • Isotype
    IgM
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt Use 5-10µl for 106 cells.

ab18400 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

WB 1/100 - 1/200.
IHC-P 1/50 - 1/100.

Target

  • Function
    Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
  • Tissue specificity
    Mainly expressed in the brain.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 43 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-glucuronyltransferase 1 antibody
    • 3-glucuronyltransferase antibody
    • B3GA1_HUMAN antibody
    • B3gat1 antibody
    • Beta 1 3 glucuronyltransferase 1 antibody
    • Beta-1 antibody
    • Beta-1,3-glucuronyltransferase 1 antibody
    • CD 57 antibody
    • CD57 antibody
    • CD57 antigen antibody
    • Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 antibody
    • Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 antibody
    • GlcAT P antibody
    • GlcAT-P antibody
    • GLCATP antibody
    • GlcUAT P antibody
    • GlcUAT-P antibody
    • GlcUATP antibody
    • Glucuronosyltransferase P antibody
    • HNK 1 antibody
    • HNK1 antibody
    • LEU 7 antibody
    • LEU7 antibody
    • LEU7 antigen antibody
    • NK 1 antibody
    • NK1 antibody
    • UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase antibody
    • UDP-GlcUA:glycoprotein beta-1 antibody
    see all

Images

  • Immunohistochemical staining of formalin-fixed paraffin-embedded Human tonsil tissue labeling CD57 with ab187274 at 1/50 dilution.

  • Flow cytometric analysis of Human lymphyocytes labeling CD57 with ab187274 with 10µl/10cells.

References

This product has been referenced in:
  • Ng SY  et al. Targetable vulnerabilities in T- and NK-cell lymphomas identified through preclinical models. Nat Commun 9:2024 (2018). Read more (PubMed: 29789628) »
  • Yu P  et al. Comparative pathology of rhesus macaque and common marmoset animal models with Middle East respiratory syndrome coronavirus. PLoS One 12:e0172093 (2017). Read more (PubMed: 28234937) »
See all 4 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spindle Cell Neoplasm human section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Spindle Cell Neoplasm human section
Blocking step
Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

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Verified customer

Submitted Jun 23 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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