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  1. Link

    cd59-antibody-epr64252-ab133707.pdf

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Immunology Cell Type Markers CD Non-lineage
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD59 antibody [EPR6425(2)] (ab133707)

  • Datasheet
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Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)
  • Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)
  • Anti-CD59 antibody [EPR6425(2)] (ab133707)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6425(2)] to CD59
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD59 antibody [EPR6425(2)]
    See all CD59 primary antibodies
  • Description

    Rabbit monoclonal [EPR6425(2)] to CD59
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HUVEC and BxPC3 cell lysates; Human placenta and tonsil tissues
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6425(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Immunology
    • Innate Immunity
    • Complement
    • MAC
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Innate and adaptive immunity

Associated products

  • Alternative Versions

    • Anti-CD59 antibody [EPR6425(2)] - BSA and Azide free (ab248625)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD59 protein (ab134887)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC or IP.

Target

  • Function

    Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
    The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
  • Involvement in disease

    Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
  • Sequence similarities

    Contains 1 UPAR/Ly6 domain.
  • Post-translational
    modifications

    N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
    Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
  • Cellular localization

    Cell membrane. Secreted. Soluble form found in a number of tissues.
  • Target information above from: UniProt accession P13987 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 966 Human
    • Omim: 107271 Human
    • SwissProt: P13987 Human
    • Unigene: 278573 Human
    • Unigene: 709466 Human
    • Unigene: 710641 Human
    • Alternative names

      • 16.3A5 antibody
      • 1F5 antibody
      • 1F5 antigen antibody
      • 20 kDa homologous restriction factor antibody
      • CD 59 antibody
      • CD_antigen=CD59 antibody
      • CD59 antibody
      • CD59 antigen antibody
      • CD59 antigen complement regulatory protein antibody
      • CD59 antigen p18 20 antibody
      • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
      • CD59 glycoprotein antibody
      • CD59 molecule antibody
      • CD59 molecule complement regulatory protein antibody
      • CD59_HUMAN antibody
      • Cd59a antibody
      • Complement regulatory protein antibody
      • EJ16 antibody
      • EJ30 antibody
      • EL32 antibody
      • FLJ38134 antibody
      • FLJ92039 antibody
      • G344 antibody
      • HRF 20 antibody
      • HRF-20 antibody
      • HRF20 antibody
      • Human leukocyte antigen MIC11 antibody
      • Ly 6 like protein antibody
      • Lymphocytic antigen CD59/MEM43 antibody
      • MAC inhibitory protein antibody
      • MAC IP antibody
      • MAC-inhibitory protein antibody
      • MAC-IP antibody
      • MACIF antibody
      • MACIP antibody
      • MEM43 antibody
      • MEM43 antigen antibody
      • Membrane attack complex (MAC) inhibition factor antibody
      • Membrane attack complex inhibition factor antibody
      • Membrane inhibitor of reactive lysis antibody
      • MGC2354 antibody
      • MIC11 antibody
      • MIN1 antibody
      • MIN2 antibody
      • MIN3 antibody
      • MIRL antibody
      • MSK21 antibody
      • p18 20 antibody
      • Protectin antibody
      • Surface antigen recognized by monoclonal antibody 16.3A5 antibody
      • T cell activating protein antibody
      see all

    Images

    • Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)
      Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)

      Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
      Lane 2: CD59 knockout HAP1 whole cell lysate (40 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab133707 observed at 14 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab133707 was shown to specifically react with CD59 in wild-type HAP1 cells as signal was lost in CD59 knockout cells. Wild-type and CD59 knockout samples were subjected to SDS-PAGE. Ab133707 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)
      Western blot - Anti-CD59 antibody [EPR6425(2)] (ab133707)
      All lanes : Anti-CD59 antibody [EPR6425(2)] (ab133707) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate
      Lane 2 : BxPC 3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 14 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)

      Immunohistochemical analysis of CD59 in paraffin embedded Human placenta tissue labelled with ab133707 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [EPR6425(2)] (ab133707)

      Immunohistochemical analysis of CD59 in paraffin embedded Human tonsil tissue labelled with ab133707 at a 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-CD59 antibody [EPR6425(2)] (ab133707)
      Anti-CD59 antibody [EPR6425(2)] (ab133707)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab133707? Please let us know so that we can cite the reference in this datasheet.

    ab133707 has been referenced in 2 publications.

    • Clark AJ & Petty HR Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy. Sci Rep 6:27039 (2016). PubMed: 27247112
    • Clark AJ & Petty HR Protocol for Biomarker Ratio Imaging Microscopy with Specific Application to Ductal Carcinoma In situ of the Breast. Front Cell Dev Biol 4:120 (2016). PubMed: 27857940

    Customer reviews and Q&As

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