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    cd59-antibody-mem-43-ab9182.pdf

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Immunology Cell Type Markers CD Non-lineage
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Anti-CD59 antibody [MEM-43] (ab9182)

  • Datasheet
  • SDS
Reviews (2)Q&A (9)References (22)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182)

    Key features and details

    • Mouse monoclonal [MEM-43] to CD59
    • Suitable for: IP, IHC-Fr, IHC, ICC/IF, Flow Cyt, WB, IHC-P
    • Reacts with: Human
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 647 APC Biotin FITC PE

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    Overview

    • Product name

      Anti-CD59 antibody [MEM-43]
      See all CD59 primary antibodies
    • Description

      Mouse monoclonal [MEM-43] to CD59
    • Host species

      Mouse
    • Specificity

      CD59 antigen (human). MEM-43 identified CD59 as the new cluster on 4th HLDA Workshop. MEM-43 reacts with well defined epitope (WhO, P-53).
    • Tested applications

      Suitable for: IP, IHC-Fr, IHC, ICC/IF, Flow Cyt, WB, IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human CD59. Thymocytes and T lymphocytes

    • Positive control

      • ICC/IF: Human fibrosarcoma cells. Flow Cyt: HT1080 cells. IHC: human placenta tissue
    • General notes

      When originally tested in WB, SDS was included in the sample buffer, however, feedback from one researcher has shown that it is best to omit SDS from the sample buffer.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Do Not Freeze.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Purity >95% by SDS-PAGE.
    • Clonality

      Monoclonal
    • Clone number

      MEM-43
    • Myeloma

      unknown
    • Isotype

      IgG2a
    • Light chain type

      unknown
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Immunology
      • Innate Immunity
      • Complement
      • MAC
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Other
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity

    Associated products

    • Alternative Versions

      • FITC Anti-CD59 antibody [MEM-43], prediluted (ab18237)
      • Alexa Fluor® 647 Anti-CD59 antibody [MEM-43], prediluted (ab187769)
      • Biotin Anti-CD59 antibody [MEM-43] (ab28114)
      • PE Anti-CD59 antibody [MEM-43] (ab36421)
      • APC Anti-CD59 antibody [MEM-43] (ab36467)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human CD59 protein (ab134887)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9182 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC
    Use a concentration of 10 µg/ml.
    ICC/IF (1)
    Use at an assay dependent concentration. PubMed: 17911601
    Flow Cyt (1)
    Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    WB
    Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

    Use under non-reducing conditions.

    IHC-P
    Use at an assay dependent concentration.
    Notes
    IP
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC
    Use a concentration of 10 µg/ml.
    ICC/IF
    Use at an assay dependent concentration. PubMed: 17911601
    Flow Cyt
    Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    WB
    Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

    Use under non-reducing conditions.

    IHC-P
    Use at an assay dependent concentration.

    Target

    • Function

      Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
      The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
    • Involvement in disease

      Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
    • Sequence similarities

      Contains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications

      N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
      Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
    • Cellular localization

      Cell membrane. Secreted. Soluble form found in a number of tissues.
    • Target information above from: UniProt accession P13987 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 966 Human
      • Omim: 107271 Human
      • SwissProt: P13987 Human
      • Unigene: 278573 Human
      • Unigene: 709466 Human
      • Unigene: 710641 Human
      • Alternative names

        • 16.3A5 antibody
        • 1F5 antibody
        • 1F5 antigen antibody
        • 20 kDa homologous restriction factor antibody
        • CD 59 antibody
        • CD_antigen=CD59 antibody
        • CD59 antibody
        • CD59 antigen antibody
        • CD59 antigen complement regulatory protein antibody
        • CD59 antigen p18 20 antibody
        • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
        • CD59 glycoprotein antibody
        • CD59 molecule antibody
        • CD59 molecule complement regulatory protein antibody
        • CD59_HUMAN antibody
        • Cd59a antibody
        • Complement regulatory protein antibody
        • EJ16 antibody
        • EJ30 antibody
        • EL32 antibody
        • FLJ38134 antibody
        • FLJ92039 antibody
        • G344 antibody
        • HRF 20 antibody
        • HRF-20 antibody
        • HRF20 antibody
        • Human leukocyte antigen MIC11 antibody
        • Ly 6 like protein antibody
        • Lymphocytic antigen CD59/MEM43 antibody
        • MAC inhibitory protein antibody
        • MAC IP antibody
        • MAC-inhibitory protein antibody
        • MAC-IP antibody
        • MACIF antibody
        • MACIP antibody
        • MEM43 antibody
        • MEM43 antigen antibody
        • Membrane attack complex (MAC) inhibition factor antibody
        • Membrane attack complex inhibition factor antibody
        • Membrane inhibitor of reactive lysis antibody
        • MGC2354 antibody
        • MIC11 antibody
        • MIN1 antibody
        • MIN2 antibody
        • MIN3 antibody
        • MIRL antibody
        • MSK21 antibody
        • p18 20 antibody
        • Protectin antibody
        • Surface antigen recognized by monoclonal antibody 16.3A5 antibody
        • T cell activating protein antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD59 antibody [MEM-43] (ab9182)

        Immunohistochemistry parafin embedded sections staining of huam palacenta tissue using ab9182 with a concentration of 10µg / ml

      Protocols

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (22)

      Publishing research using ab9182? Please let us know so that we can cite the reference in this datasheet.

      ab9182 has been referenced in 22 publications.

      • Brandel A  et al. The Gb3-enriched CD59/flotillin plasma membrane domain regulates host cell invasion by Pseudomonas aeruginosa. Cell Mol Life Sci 78:3637-3656 (2021). PubMed: 33555391
      • Yan B  et al. Effects of the Bone/Bone Marrow Microenvironments on Prostate Cancer Cells and CD59 Expression. Biomed Res Int 2020:2753414 (2020). PubMed: 32337233
      • Kalappurakkal JM  et al. Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading. Cell 177:1738-1756.e23 (2019). PubMed: 31104842
      • Yan B  et al. iTRAQ-based Comparative Serum Proteomic Analysis of Prostate Cancer Patients with or without Bone Metastasis. J Cancer 10:4165-4177 (2019). PubMed: 31413735
      • Chen Y  et al. Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds. J Cell Mol Med 23:5956-5969 (2019). PubMed: 31293077
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

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      1-2 of 2 Abreviews

      Flow Cytometry abreview for Anti-CD59 antibody [MEM-43]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (Fibrosarcoma (HT1080))
      Specification
      Fibrosarcoma (HT1080)
      Fixation
      Paraformaldehyde
      Permeabilization
      No
      Gating Strategy
      no gating
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 13 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD59 antibody [MEM-43]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (fibrosarcoma)
      Specification
      fibrosarcoma
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 19 2008

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