Overview

  • Product name
    Anti-CD59 antibody [YTH53.1] (FITC)
    See all CD59 primary antibodies
  • Description
    Rat monoclonal [YTH53.1] to CD59 (FITC)
  • Host species
    Rat
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody is specific for CD59.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Peripheral Blood T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab33150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: 1/1.
    Use 10ul of neat antibody to label 106 cells in 100ul.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
      The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
    • Involvement in disease
      Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
    • Sequence similarities
      Contains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications
      N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
      Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
    • Cellular localization
      Cell membrane. Secreted. Soluble form found in a number of tissues.
    • Information by UniProt
    • Database links
    • Alternative names
      • 16.3A5 antibody
      • 1F5 antibody
      • 1F5 antigen antibody
      • 20 kDa homologous restriction factor antibody
      • CD 59 antibody
      • CD_antigen=CD59 antibody
      • CD59 antibody
      • CD59 antigen antibody
      • CD59 antigen complement regulatory protein antibody
      • CD59 antigen p18 20 antibody
      • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
      • CD59 glycoprotein antibody
      • CD59 molecule antibody
      • CD59 molecule complement regulatory protein antibody
      • CD59_HUMAN antibody
      • Cd59a antibody
      • Complement regulatory protein antibody
      • EJ16 antibody
      • EJ30 antibody
      • EL32 antibody
      • FLJ38134 antibody
      • FLJ92039 antibody
      • G344 antibody
      • HRF 20 antibody
      • HRF-20 antibody
      • HRF20 antibody
      • Human leukocyte antigen MIC11 antibody
      • Ly 6 like protein antibody
      • Lymphocytic antigen CD59/MEM43 antibody
      • MAC inhibitory protein antibody
      • MAC IP antibody
      • MAC-inhibitory protein antibody
      • MAC-IP antibody
      • MACIF antibody
      • MACIP antibody
      • MEM43 antibody
      • MEM43 antigen antibody
      • Membrane attack complex (MAC) inhibition factor antibody
      • Membrane attack complex inhibition factor antibody
      • Membrane inhibitor of reactive lysis antibody
      • MGC2354 antibody
      • MIC11 antibody
      • MIN1 antibody
      • MIN2 antibody
      • MIN3 antibody
      • MIRL antibody
      • MSK21 antibody
      • p18 20 antibody
      • Protectin antibody
      • Surface antigen recognized by monoclonal antibody 16.3A5 antibody
      • T cell activating protein antibody
      see all

    References

    This product has been referenced in:
    • Brasoveanu LI  et al. Melanoma cells constitutively release an anchor-positive soluble form of protectin (sCD59) that retains functional activities in homologous complement-mediated cytotoxicity. J Clin Invest 100:1248-55 (1997). Read more (PubMed: 9276743) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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