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    cd62e-antibody-12b6-ab6630.pdf

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Immunology Cell Type Markers CD Adhesion
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Anti-CD62E antibody [1.2B6] (ab6630)

  • Datasheet
  • SDS
Reviews (3)Q&A (5)References (11)

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Flow Cytometry - Anti-CD62E antibody [1.2B6] (ab6630)

    Key features and details

    • Mouse monoclonal [1.2B6] to CD62E
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    Protein
    Product image
    Recombinant Human CD62E protein (ab167744)
    Secondary
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    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Primary
    Biotin Anti-C5b-9 + C5b-8 antibody [aE11] (ab237699)

    View more associated products

    Overview

    • Product name

      Anti-CD62E antibody [1.2B6]
      See all CD62E primary antibodies
    • Description

      Mouse monoclonal [1.2B6] to CD62E
    • Host species

      Mouse
    • Specificity

      This antibody recognises the human CD62E and CD62P cell surface antigens binding to a common epitope on these members of the selectin family.
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      Human E-Selectin (ELAM-1)

    • General notes


      This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
    • Primary antibody notes

      This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.
    • Clonality

      Monoclonal
    • Clone number

      1.2B6
    • Myeloma

      NS1
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Leukocyte recruitment
      • Cell adhesion molecules
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Endothelial Cells
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human CD62E protein (ab167744)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6630 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    Application Abreviews Notes
    Flow Cyt
    Use a concentration of 10 µg/ml.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use a concentration of 10 µg/ml.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
    • Sequence similarities

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P16581 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6401 Human
      • Omim: 131210 Human
      • SwissProt: P16581 Human
      • Unigene: 82848 Human
      • Alternative names

        • CD26E antibody
        • CD62 antigen-like family member E antibody
        • CD62E antibody
        • E selectin antibody
        • E-selectin antibody
        • ELAM antibody
        • ELAM-1 antibody
        • ELAM1 antibody
        • Endothelial adhesion molecule 1 antibody
        • Endothelial leukocyte adhesion molecule 1 antibody
        • ESEL antibody
        • LECAM2 antibody
        • Leukocyte-endothelial cell adhesion molecule 2 antibody
        • LYAM2_HUMAN antibody
        • Sele antibody
        • Selectin E antibody
        see all

      Images

      • Flow Cytometry - Anti-CD62E antibody [1.2B6] (ab6630)
        Flow Cytometry - Anti-CD62E antibody [1.2B6] (ab6630)
        ab6630 staining CD62E in thrombin activated human peripheral blood platelets by Flow Cytometry analysis.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (11)

      Publishing research using ab6630? Please let us know so that we can cite the reference in this datasheet.

      ab6630 has been referenced in 11 publications.

      • Yuan X  et al. Recipient treatment with acetylcholinesterase inhibitor donepezil attenuates primary graft failure in rats through inhibiting post-transplantational donor heart ischaemia/reperfusion injury. Eur J Cardiothorac Surg 53:400-408 (2018). PubMed: 28950359
      • Zhang X  et al. Long Noncoding RNA Malat1 Regulates Cerebrovascular Pathologies in Ischemic Stroke. J Neurosci 37:1797-1806 (2017). PubMed: 28093478
      • Zhang XJ  et al. shRNAs targeting high-mobility group box-1 inhibit E-selectin expression via homeobox A9 in human umbilical vein endothelial cells. Mol Med Rep 7:1251-6 (2013). PubMed: 23403989
      • Allen CL  et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). PubMed: 24205233
      • Zhou H  et al. NAD(P)H:quinone oxidoreductase 1-compromised human bone marrow endothelial cells exhibit decreased adhesion molecule expression and CD34+ hematopoietic cell adhesion. J Pharmacol Exp Ther 334:260-8 (2010). WB ; Human . PubMed: 20378716
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-8 of 8 Abreviews or Q&A

      Question

      Hi Abcam,
      I have received the following enquiry from a customer, please see below;
      AB955 – Anti-CD68
      AB81720 – Anti-CD73
      AB95139 – Anti-VCAM1
      AB6630 – Anti-CD62E (E-selectin)
      I have also read the fact sheets for all, but to clarify, they are all suitable for IHC using frozen sections fixed with PFA?
      Could you please confirm the above?
      Thanks for your help!
      Kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on May 01 2012

      Answer

      Thank you for contacting us.

      Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

      However, instead of using PFA as a fixative, I would better suggest using acetone, methanol or ethanol at -20°C for 30 min, so the additional antigen retrieval step can be skipped. If, by the tissue’s nature PFA is required as a fixative, antigen retrieval is recommended to allow the antibody reach the epitope.

      All of our products are covered by our Abpromise® guarantee, which ensures that you can trust them, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

      To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on May 01 2012

      Western blot abreview for Anti-CD62E antibody [1.2B6]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (human nasal tissue)
      Loading amount
      90 µg
      Specification
      human nasal tissue
      Gel Running Conditions
      Non-reduced Denaturing (10 %)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
      Read More

      Mr. Michael Könnecke

      Verified customer

      Submitted Jan 11 2013

      Question

      I have a question regarding your anti-CD62E (E-selectin) antibodies, such as ab6630.  Since E-selectin is localized to the cell surface/membrane, there should be no need to permeabilize cells for an immunocytochemical stain.  Is this correct?  Or is permeabilization necessary?   Also, can you recommend a polyclonal anti-CD62E antibody that would be appropriate for whole mount fluorescence staining of human endothelial cells?   Thank you for your help.  

      Read More

      Abcam community

      Verified customer

      Asked on Jun 22 2012

      Answer

      Thank you for contacting us.

      You are quite correct. As CD62E is a single pass membrane bound protein, there should be no need to permeabilze the cells for an immunocytochemical stain.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Jun 22 2012

      ELISA abreview for Anti-CD62E antibody [1.2B6]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Cell (HUVEC)
      Specification
      HUVEC
      Type
      Direct
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 07 2011

      Immunohistochemistry (Frozen sections) abreview for Anti-CD62E antibody [1.2B6]

      Good
      Abreviews
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Intestine)
      Specification
      Intestine
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - Citrate Buffer
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 20 2007

      Question

      I have a question about you ICAM-1(ab2213), VCAM-1(ab19264) and ELAM-1(ab6630) primary antibodies. I am using a cell dissociation solution to (P-8 -- P-10) HUVECs from a T-75 tissue culture flash, normally takes about 10mins. after removal the cells are examined for presence of ICAM-1, VCAM-1 and ELAM-1 using antibody staining and FACs. I have used these antibodies at various concentrations (1ug/ul, 2.5ug/ul & 5ug/ul). the secondary antibodies used are IgG2a and IgG1 isotype and FITC conjugated (BD biosciences, 553456, 550616) respectively. these secondary antibodies have been optimised to prevent non specific binding. the primaries antibodies are incubated with the cells (30,000 ECs/well) for 30mins on ice. the cells are fixed with 4% PFA at the end of the protocol. to date I have not been able to optimise the primary antibody concentrations to give good results, i.e. a definite increase in secondary fluorescence compared to cells alone with no primary antibody and no secondary antibody. I would like to know if you know of any optimal conc. to use each of the primary antibodies and what addition steps that could be used to improve my results. it is possible that the cell dissociation solution is destroying any present adhesion molecules.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 28 2005

      Answer

      I have received feedback from the source of ab6630 and ab19264 and would like to share their feedback. Unfortunately I am still awaiting feedback regarding ab2213. For ab6630 we would suggest a working dilution of 10ug/ml. For ab19264 we recommend to use 0.5 - 1µg antibody for 106 cells(I am still awaiting feedback from the source of ab2213 to find out the recommended dilution). We think it is indeed very likely that the "cell dissociation solution" is either removing or damaging these cell adhesion molecules on HUVECs. It is not clear what this solution is, but if it is trypsin or something similar to trypsin, this could be the source of your problem. HUVECs should be positive for ICAM-1 and VCAM-1, but the expression of those proteins can be low if the cells aren't stimulated by inflammatory mediators. Furthermore, we do not know if HUVECs express ELAM-1 unless stimulated in the appropriate manner. The following reference may help: Schatz F, Soderland C, Hendricks-Munoz KD, Gerrets RP, Lockwood CJ. Human endometrial endothelial cells: isolation, characterization, and inflammatory-mediated expression of tissue factor and type 1 plasminogen activator inhibitor. Biol Reprod. 2000 Mar;62(3):691-7. I would like to suggest that you try mixing the primary antibodies with your cells at room temp instead of on ice and as soon as I find out the recommended dilution for ab2213 I will let you know, thank you for your patience.

      Read More

      Abcam Scientific Support

      Answered on Nov 01 2005

      Question

      I just wonder why your antibody (selectin E-ab6630) only works under non-reduced condition in Western blotting. What is your "non-reduced" condition.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 16 2001

      Answer

      The antibody will recognise the native form of the epitope thus Westerns must be conducted in non-reducing conditions (i.e. without DTT/ beta-mercaptoethanol

      Read More

      Abcam Scientific Support

      Answered on Oct 17 2001

      Question

      Is there a protocol for immunohistochemistry available for this antibody? We are interested in using an antibody to E-Selectin in human endometrial tissue that is formalin-fixed and paraffin embedded. We want to know if there are any published results using this exact antibody for immunohistochemistry on any tissue. What is a good positive control tissue? Where abouts in the cell is the staining localised? Do you supply free samples for trial, or sell small quantities for trial?

      Read More

      Abcam community

      Verified customer

      Asked on May 02 2001

      Answer

      The antibody has been characterised extensively and published in: J Immunol 1990 Apr 1;144(7):2558-65 A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. Wellicome SM, Thornhill MH, Pitzalis C, Thomas DS, Lanchbury JS, Panayi GS, Haskard DO.
      and
      J Immunol 1990 Aug 1;145(3):865-72 IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor, or IFN-gamma. Thornhill MH, Haskard DO.
      It's use in immunohistochemstry has been described in: Am J Physiol 1994 Jan;266(1 Pt 2):H278- Characterization of E-selectin expression in vivo with use of a radiolabeled monoclonal antibody. Keelan ET, Licence ST, Peters AM, Binns RM, Haskard DO.

      Read More

      Abcam Scientific Support

      Answered on May 03 2001

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