Overview

  • Product name
    Anti-CD62E antibody [1.2B6]
    See all CD62E primary antibodies
  • Description
    Mouse monoclonal [1.2B6] to CD62E
  • Host species
    Mouse
  • Specificity
    This antibody recognises the human CD62E and CD62P cell surface antigens binding to a common epitope on these members of the selectin family.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, IHC-Fr, IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Human E-Selectin (ELAM-1)

  • General notes


    This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab6630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Non-reduced only.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
    • Sequence similarities
      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • CD26E antibody
        • CD62 antigen-like family member E antibody
        • CD62E antibody
        • E selectin antibody
        • E-selectin antibody
        • ELAM antibody
        • ELAM-1 antibody
        • ELAM1 antibody
        • Endothelial adhesion molecule 1 antibody
        • Endothelial leukocyte adhesion molecule 1 antibody
        • ESEL antibody
        • LECAM2 antibody
        • Leukocyte-endothelial cell adhesion molecule 2 antibody
        • LYAM2_HUMAN antibody
        • Sele antibody
        • Selectin E antibody
        see all

      Images

      • Flow Cytometry - Anti-CD62E antibody [1.2B6] (ab6630)
        Flow Cytometry - Anti-CD62E antibody [1.2B6] (ab6630)
        ab6630 staining CD62E in thrombin activated human peripheral blood platelets by Flow Cytometry analysis.

      References

      This product has been referenced in:

      See all 9 Publications for this product

      Publishing research using ab6630? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      8 reviews or Q&A

      10
      Votes: Highest First

      Hi Abcam,
      I have received the following enquiry from a customer, please see below;
      AB955 – Anti-CD68
      AB81720 – Anti-CD73
      AB95139 – Anti-VCAM1
      AB6630 – Anti-CD62E (E-selectin)
      I have also read the fa...

      Read More

      Thank you for contacting us.

      Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

      However, instead of using PFA as a fixative, I w...

      Read More

      Western blot abreview for Anti-CD62E antibody [1.2B6]

       Average
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (human nasal tissue)
      Loading amount
      90 µg
      Specification
      human nasal tissue
      Gel Running Conditions
      Non-reduced Denaturing (10 %)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
      Read More
      abreview image
      Username

      Mr. Michael Könnecke

      Verified customer

      Submitted Jan 11 2013

      I have a question regarding your anti-CD62E (E-selectin) antibodies, such as ab6630.  Since E-selectin is localized to the cell surface/membrane, there should be no need to permeabilize cells for an immunocytochemical stain.  Is this correct?  Or is pe...

      Read More

      Thank you for contacting us.

      You are quite correct. As CD62E is a single pass membrane bound protein, there should be no need to permeabilze the cells for an immunocytochemical stain.

      I hope this information is helpful to you....

      Read More

      ELISA abreview for Anti-CD62E antibody [1.2B6]

       Average
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      ELISA
      Sample
      Human Cell (HUVEC)
      Specification
      HUVEC
      Type
      Direct
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Jun 07 2011

      Immunohistochemistry (Frozen sections) abreview for Anti-CD62E antibody [1.2B6]

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Intestine)
      Specification
      Intestine
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - Citrate Buffer
      Read More
      Username

      Abcam user community

      Verified customer

      Submitted Aug 20 2007

      I have a question about you ICAM-1(ab2213), VCAM-1(ab19264) and ELAM-1(ab6630) primary antibodies. I am using a cell dissociation solution to (P-8 -- P-10) HUVECs from a T-75 tissue culture flash, normally takes about 10mins. after removal the cells...

      Read More

      I have received feedback from the source of ab6630 and ab19264 and would like to share their feedback. Unfortunately I am still awaiting feedback regarding ab2213. For ab6630 we would suggest a working dilution of 10ug/ml. For ab19264 we recommend...

      Read More

      I just wonder why your antibody (selectin E-ab6630) only works under non-reduced condition in Western blotting. What is your "non-reduced" condition.

      The antibody will recognise the native form of the epitope thus Westerns must be conducted in non-reducing conditions (i.e. without DTT/ beta-mercaptoethanol

      Is there a protocol for immunohistochemistry available for this antibody? We are interested in using an antibody to E-Selectin in human endometrial tissue that is formalin-fixed and paraffin embedded. We want to know if there are any published res...

      Read More

      The antibody has been characterised extensively and published in: J Immunol 1990 Apr 1;144(7):2558-65 A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. Well...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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