Overview

  • Product name
    Anti-CD62E antibody [1.2B6]
    See all CD62E primary antibodies
  • Description
    Mouse monoclonal [1.2B6] to CD62E
  • Host species
    Mouse
  • Specificity
    This antibody recognises the human CD62E and CD62P cell surface antigens binding to a common epitope on these members of the selectin family.
  • Tested applications
    Suitable for: ELISA, Flow Cyt, IHC-Fr, IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Human E-Selectin (ELAM-1)

  • General notes


    This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab6630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Non-reduced only.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
    • Sequence similarities
      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD26E antibody
      • CD62 antigen-like family member E antibody
      • CD62E antibody
      • E selectin antibody
      • E-selectin antibody
      • ELAM antibody
      • ELAM-1 antibody
      • ELAM1 antibody
      • Endothelial adhesion molecule 1 antibody
      • Endothelial leukocyte adhesion molecule 1 antibody
      • ESEL antibody
      • LECAM2 antibody
      • Leukocyte-endothelial cell adhesion molecule 2 antibody
      • LYAM2_HUMAN antibody
      • Sele antibody
      • Selectin E antibody
      see all

    Images

    • ab6630 staining CD62E in thrombin activated human peripheral blood platelets by Flow Cytometry analysis.

    References

    This product has been referenced in:
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-8 of 8 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

    However, instead of using PFA as a fixative, I would better suggest using acetone, methanol or ethanol at -20°C for 30 min, so the additional antigen retrieval step can be skipped. If, by the tissue’s nature PFA is required as a fixative, antigen retrieval is recommended to allow the antibody reach the epitope.

    All of our products are covered by our Abpromise® guarantee, which ensures that you can trust them, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

    To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (human nasal tissue)
    Loading amount
    90 µg
    Specification
    human nasal tissue
    Gel Running Conditions
    Non-reduced Denaturing (10 %)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

    Mr. Michael Könnecke

    Verified customer

    Submitted Jan 11 2013

    Answer

    Thank you for contacting us.

    You are quite correct. As CD62E is a single pass membrane bound protein, there should be no need to permeabilze the cells for an immunocytochemical stain.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Cell (HUVEC)
    Specification
    HUVEC
    Type
    Direct
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Jun 07 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Intestine)
    Specification
    Intestine
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Citrate Buffer

    Abcam user community

    Verified customer

    Submitted Aug 20 2007

    Answer

    I have received feedback from the source of ab6630 and ab19264 and would like to share their feedback. Unfortunately I am still awaiting feedback regarding ab2213. For ab6630 we would suggest a working dilution of 10ug/ml. For ab19264 we recommend to use 0.5 - 1µg antibody for 106 cells(I am still awaiting feedback from the source of ab2213 to find out the recommended dilution). We think it is indeed very likely that the "cell dissociation solution" is either removing or damaging these cell adhesion molecules on HUVECs. It is not clear what this solution is, but if it is trypsin or something similar to trypsin, this could be the source of your problem. HUVECs should be positive for ICAM-1 and VCAM-1, but the expression of those proteins can be low if the cells aren't stimulated by inflammatory mediators. Furthermore, we do not know if HUVECs express ELAM-1 unless stimulated in the appropriate manner. The following reference may help: Schatz F, Soderland C, Hendricks-Munoz KD, Gerrets RP, Lockwood CJ. Human endometrial endothelial cells: isolation, characterization, and inflammatory-mediated expression of tissue factor and type 1 plasminogen activator inhibitor. Biol Reprod. 2000 Mar;62(3):691-7. I would like to suggest that you try mixing the primary antibodies with your cells at room temp instead of on ice and as soon as I find out the recommended dilution for ab2213 I will let you know, thank you for your patience.

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    Answer

    The antibody will recognise the native form of the epitope thus Westerns must be conducted in non-reducing conditions (i.e. without DTT/ beta-mercaptoethanol

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    Answer

    The antibody has been characterised extensively and published in: J Immunol 1990 Apr 1;144(7):2558-65 A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. Wellicome SM, Thornhill MH, Pitzalis C, Thomas DS, Lanchbury JS, Panayi GS, Haskard DO.
    and
    J Immunol 1990 Aug 1;145(3):865-72 IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor, or IFN-gamma. Thornhill MH, Haskard DO.
    It's use in immunohistochemstry has been described in: Am J Physiol 1994 Jan;266(1 Pt 2):H278- Characterization of E-selectin expression in vivo with use of a radiolabeled monoclonal antibody. Keelan ET, Licence ST, Peters AM, Binns RM, Haskard DO.

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