Overview

  • Product name
    Anti-CD62E antibody
    See all CD62E primary antibodies
  • Description
    Rabbit polyclonal to CD62E
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide surrounding aa 584 of human CD62E

  • Positive control
    • Jurkat cell lysate, mouse small intestine and rat kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab18981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 67 kDa.Can be blocked with CD62E peptide (ab113900).

Target

  • Function
    Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • CD26E antibody
    • CD62 antigen-like family member E antibody
    • CD62E antibody
    • E selectin antibody
    • E-selectin antibody
    • ELAM antibody
    • ELAM-1 antibody
    • ELAM1 antibody
    • Endothelial adhesion molecule 1 antibody
    • Endothelial leukocyte adhesion molecule 1 antibody
    • ESEL antibody
    • LECAM2 antibody
    • Leukocyte-endothelial cell adhesion molecule 2 antibody
    • LYAM2_HUMAN antibody
    • Sele antibody
    • Selectin E antibody
    see all

Images

  • Western blot - Anti-CD62E antibody (ab18981)
    Western blot - Anti-CD62E antibody (ab18981)
    Anti-CD62E antibody (ab18981) + Jurkat cell lysate

    Predicted band size: 67 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)

    ab18981 IHC staining of mouse aorta from obese diabetic mice with E-Selectin Polyclonal antibody: Immunohistochemistry of paraffin-embedded tissue after heat-induced antigen retrieval. 10-20 μg/mL of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit HRP and visualized with DAB substrate using ABC staining system.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)

    ab18981 IHC staining of human placenta with E-Selectin Polyclonal antibody: Immunohistochemistry of formalin fixed, paraffin-embedded tissue after heat-induced antigen retrieval. 5 μg/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

  • Western blot - Anti-CD62E antibody (ab18981)
    Western blot - Anti-CD62E antibody (ab18981)
    Anti-CD62E antibody (ab18981) at 2 µg/ml + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody (ab18981)
    Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody (ab18981)This image is courtesy of an anonymous Abreview

    ab18981 staining CD62E in Rat brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in 1% BSA) for 18 hours at 4°C. A donkey anti-rabbit DyLight® 650 (IgG; 1/500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • García Nores GD  et al. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema. Nat Commun 9:1970 (2018). Read more (PubMed: 29773802) »
  • Zhang X  et al. Long Noncoding RNA Malat1 Regulates Cerebrovascular Pathologies in Ischemic Stroke. J Neurosci 37:1797-1806 (2017). Read more (PubMed: 28093478) »

See all 17 Publications for this product

Publishing research using ab18981? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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8 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD62E antibody

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293-T)
Permeabilization
Yes - .1% triton
Specification
293-T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
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Verified customer

Submitted Nov 30 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD62E antibody

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Rat Cell (Rat Brain Microvascular Endothelial Cells (RBMVEC))
Specification
Rat Brain Microvascular Endothelial Cells (RBMVEC)
Permeabilization
No
Fixative
Paraformaldehyde
Read More
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Abcam user community

Verified customer

Submitted Nov 19 2013

Please provide the IHC protocol

1. Deparaffinize/hydrate sections:
a) Incubate sections in three washes of xylene for 5 minutes each.
b) Incubate sections in two washes of 100% ethanol for 10 minutes each.
c) Incubate sections in two washes of 95% ethanol for 10 minute...

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Dear,
I ordered the following antibodies and received them at the end of September:
- Ab54426 (anti-CD62P)
- Ab91353 (Mouse IgG1 – isotype control)
- Ab 18981 (anti-CD62E)
- Ab27478 (rabbit polyclonal IgG)
- Ab2213 (...

Read More

Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

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Dear,
We want to proceed and test ab54426 in IHC-P. You can send me the discount code.
Are these the correct isotype antibodies for the primary antibodies I have chosen:
- Mouse IgG2a, kappa monoclonal [MG2a-53] - isotype control (Ab1841...

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I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

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WB with HUVEC cells
sees band at 35 kDa instead of 67 kDa
also high background
Ab: 4 ug/ml
2nd: usually OK
block: 5% milk
not sure if reducing conditions were used
suggestions: use less 1st and 2nd Abs, add reducing a...

Read More

Thank you for contacting us.
I am sorry to hear that the antibody does not seem to work as expected.

As we had discussed, I would suggest to try the following tips to reduce the background as well as shift the MW to a higher band:
<...

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Thank you for your quick reply.

Yes, we have the band in 67kDa, too.

However we don’t have the negative control because our sample is the whole brain of rats. We sacrificed the rats and took out the brain, then homogeni...

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I agree with Heather that it would be useful to have a negative control sample. I may be mistaken but if E-selectin is only expressed exclusively in endothelial cells, perhaps using an extract from a different cell type as a negative control ...

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WB: sees 3 bands peptide + Ab (o/n, 4 degree): no band diluted Ab (as control, o/n, 4 degree): also no band --> probably due to absorption of Ab to tube wall peptide + Ab (30 min, RT): same bands as with Ab only --> probably binding equil. not ...

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Thank you for your phone call. I just wanted to follow up with you and send you the suggestions we had discussed over the phone: 1) use peptide in 10-fold excess 2) incubate peptide with Ab for 1-2 h at RT before using for WB 3) avoid prepa...

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