For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide surrounding aa 584 of human CD62E
Our Abpromise guarantee covers the use of ab18981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 67 kDa.Can be blocked with CD62E peptide (ab113900).|
ab18981 IHC staining of mouse aorta from obese diabetic mice with E-Selectin Polyclonal antibody: Immunohistochemistry of paraffin-embedded tissue after heat-induced antigen retrieval. 10-20 μg/mL of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit HRP and visualized with DAB substrate using ABC staining system.
ab18981 IHC staining of human placenta with E-Selectin Polyclonal antibody: Immunohistochemistry of formalin fixed, paraffin-embedded tissue after heat-induced antigen retrieval. 5 μg/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
ab18981 staining CD62E in Rat brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in 1% BSA) for 18 hours at 4°C. A donkey anti-rabbit DyLight® 650 (IgG; 1/500) was used as the secondary antibody.