Anti-CD62E antibody (ab18981)
Key features and details
- Rabbit polyclonal to CD62E
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CD62E antibody
See all CD62E primary antibodies -
Description
Rabbit polyclonal to CD62E -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P MouseHumanWB Human -
Immunogen
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Positive control
- WB: Jurkat cell lysate. Flow Cyt: Jurkat cells. IHC-P: Human placenta and mouse aorta tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.15% EDTA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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IHC-P |
Mouse
Human
|
WB |
Human
|
All applications |
Rat
|
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 67 kDa.Can be blocked with CD62E peptide (ab113900).
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|
Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 67 kDa.Can be blocked with CD62E peptide (ab113900). |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6401 Human
- Entrez Gene: 20339 Mouse
- Entrez Gene: 25544 Rat
- Omim: 131210 Human
- SwissProt: P16581 Human
- SwissProt: Q00690 Mouse
- SwissProt: P98105 Rat
- Unigene: 82848 Human
see all -
Alternative names
- CD26E antibody
- CD62 antigen-like family member E antibody
- CD62E antibody
see all
Images
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Anti-CD62E antibody (ab18981) at 1/1000 dilution + Jurkat cell lysate at 80 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaBlocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST.
Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)
ab18981 IHC staining of human placenta with E-Selectin Polyclonal antibody: Immunohistochemistry of formalin fixed, paraffin-embedded tissue after heat-induced antigen retrieval. 5 μg/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
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Flow cytometric analysis of Jurkat cells using ab18981 (red) with negative controls (black and green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)
ab18981 IHC staining of mouse aorta from obese diabetic mice with E-Selectin Polyclonal antibody: Immunohistochemistry of paraffin-embedded tissue after heat-induced antigen retrieval. 10-20 μg/mL of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated goat anti-rabbit HRP and visualized with DAB substrate using ABC staining system.
Protocols
References (26)
ab18981 has been referenced in 26 publications.
- Zhang K et al. Activation of brain endothelium by Escherichia coli K1 virulence factor cglD promotes polymorphonuclear leukocyte transendothelial migration. Med Microbiol Immunol 208:59-68 (2019). PubMed: 30171337
- Takeda Y et al. ROCK2 Regulates Monocyte Migration and Cell to Cell Adhesion in Vascular Endothelial Cells. Int J Mol Sci 20:N/A (2019). PubMed: 30884801
- Wang M et al. Role of TLR-4 in anti-ß2-glycoprotein I-induced activation of peritoneal macrophages and vascular endothelial cells in mice. Mol Med Rep 19:4353-4363 (2019). PubMed: 30942412
- Xu G et al. Dose-Dependent Influences of Ethanol on Ischemic Stroke: Role of Inflammation. Front Cell Neurosci 13:6 (2019). PubMed: 30853895
- Wu Y et al. Silencing of RAD51AP1 suppresses epithelial-mesenchymal transition and metastasis in non-small cell lung cancer. Thorac Cancer 10:1748-1763 (2019). PubMed: 31317661