Overview

  • Product name
    Anti-CD62E antibody [ENA1] (Biotin)
    See all CD62E primary antibodies
  • Description
    Mouse monoclonal [ENA1] to CD62E (Biotin)
  • Host species
    Mouse
  • Conjugation
    Biotin
  • Specificity
    This antibody reacts with human endothelial cells activated with TNF, IL1 or endotoxin. It was found to react also with cells transfected with the the E-selectin gene.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Tissue/ cell preparation containing human CD3

  • Positive control
    • Endothelial cells or a small fresh skin biopsy after incubation with TNF, IL1 or LPS.
  • General notes


    This antibody is useful for the staining of CD62E expressing endothelial cells. This antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.

Properties

Applications

Our Abpromise guarantee covers the use of ab36688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Fixed with acetone, followed by incubation for 10 min in chloroform.

ICC/IF Use at an assay dependent concentration.

Fixed with paraformaldehyde.

Target

  • Function
    Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD26E antibody
    • CD62 antigen-like family member E antibody
    • CD62E antibody
    • E selectin antibody
    • E-selectin antibody
    • ELAM antibody
    • ELAM-1 antibody
    • ELAM1 antibody
    • Endothelial adhesion molecule 1 antibody
    • Endothelial leukocyte adhesion molecule 1 antibody
    • ESEL antibody
    • LECAM2 antibody
    • Leukocyte-endothelial cell adhesion molecule 2 antibody
    • LYAM2_HUMAN antibody
    • Sele antibody
    • Selectin E antibody
    see all

References

This product has been referenced in:
  • Leeuwenberg JF  et al. Adhesion of polymorphonuclear cells to human endothelial cells. Adhesion-molecule-dependent, and Fc receptor-mediated adhesion-molecule-independent mechanisms. Clin Exp Immunol 81:496-500 (1990). Read more (PubMed: 1697800) »
  • Leeuwenberg JF  et al. Functional polymorphism of IgG FcRII (CD32) on human neutrophils. Immunology 71:301-4 (1990). Read more (PubMed: 2146215) »
See all 3 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you very much for your call yesterday.

We have many anti E-selectin and VCAM1 antibodies in catalogue. Please click the link below to select;

https://www.abcam.com/index.html?pageconfig=searchresults&search=e selectin&pt=1&sk=targ&sv=CD62E&sn=CD62E&l=1&fViewMore=1

https://www.abcam.com/index.html?pageconfig=searchresults&search=VCAM1&pt=1&sk=targ&sv=VCAM1&sn=VCAM1&l=1&fViewMore=1

Unfortunately none of these antibodies are tested or predicted for bovine VCAM1 and anti E-selectin cross reactivity. I am sorry we are unable to recommend any product. The homology sequence of bovine (http://www.uniprot.org/uniprot/P98107) and human (http://www.uniprot.org/uniprot/P16581) proteins are quite random; there are fragments of high and low homology which makes selection of antibodies difficult.

http://www.uniprot.org/uniprot/A7MBB0and http://www.uniprot.org/uniprot/P19320

I am sorry we are unable to suggest any tested products that will detect the bovine protein. You may however try the antibodies against the full length protein that may pick up the bovine protein.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions or concerns.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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