Product nameAnti-CD62E antibody [ENA2]
See all CD62E primary antibodies
DescriptionMouse monoclonal [ENA2] to CD62E
SpecificityReacts with E-selectin CD62-E, previously designated the Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1)[1-4]. The antibody reacts with human endothelial cells activated with TNFalpha, IL-1 or endotoxin. The antibody was found to react also with cells transfected with the E-selectin gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic. This antibody do not cross react with P-Selectin.
Tested applicationsSuitable for: IHC-Fr, Agglutination, ELISAmore details
Species reactivityReacts with: Human, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey
General notesF(ab')2 fragmented antibody
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab8165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration.
Agg: Use at an assay dependent dilution.
The antibody is extremely useful for staining of E-selectin expressing endothelial cells. It permits staining of in vitro cultured cells as well as frozen sections. For adhesion inhibition studies the F(ab')2 ENA2 monoclonal antibody preparation is most efficient. Use of F(ab')2 prevents adhesion of leucocytes via Fc receptors (2).
Instructions for use:
In vitro cultured cells can be fixed with 1% paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNFalpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 ¥ìg/ml) in tissue culture medium at 37¢ªC. As negative control it is advised to use a control murine IgG1 antibody. For inhibition studies care should be taken to incubate endothelial cells before addition of granulocytes or to use higher concentrations of antibody in case the antibody is added simultaneously.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
Sequence similaritiesBelongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains.
- Information by UniProt
- CD26E antibody
- CD62 antigen-like family member E antibody
- CD62E antibody
ab8165 has not yet been referenced specifically in any publications.