Overview

  • Product name
    Anti-CD62E antibody [UZ6]
    See all CD62E primary antibodies
  • Description
    Rat monoclonal [UZ6] to CD62E
  • Host species
    Rat
  • Tested applications
    Suitable for: ICC/IF, ELISA, Flow Cyt, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab2497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 67 kDa.

Target

  • Function
    Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD26E antibody
    • CD62 antigen-like family member E antibody
    • CD62E antibody
    • E selectin antibody
    • E-selectin antibody
    • ELAM antibody
    • ELAM-1 antibody
    • ELAM1 antibody
    • Endothelial adhesion molecule 1 antibody
    • Endothelial leukocyte adhesion molecule 1 antibody
    • ESEL antibody
    • LECAM2 antibody
    • Leukocyte-endothelial cell adhesion molecule 2 antibody
    • LYAM2_HUMAN antibody
    • Sele antibody
    • Selectin E antibody
    see all

References

This product has been referenced in:
  • Sapieha P  et al. 5-Lipoxygenase metabolite 4-HDHA is a mediator of the antiangiogenic effect of ?-3 polyunsaturated fatty acids. Sci Transl Med 3:69ra12 (2011). Read more (PubMed: 21307302) »
  • Hammel M  et al. Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion. Exp Cell Res 269:266-74 (2001). Read more (PubMed: 11570819) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thanks you for your email.
In my previous email there is a correction about the expiry date. The expiry date should be read as 25-08-2012.
I have asked lab to release the immunogen information of these antibodies; I will be able to send the answer to your question soon.
Many thanks for having patience!

Read More

Answer

Thank you very much for your email!

I hope you are well as well! Here we are fine - we have one of our first days of spring :-)

In regards to your questions:

The CD62E antibodies ab22631 et ab2497 are two different clones. Indeed, both are monoclonal rat antibodies, and I would think from the same fusion/productions, however they are two distinct antibodies - once clone UY7 and once clone UZ6. as therefore the epitope is most likely also different, they need therefore to be tested separately for all applications and species reactivity. Please let me know if you would need any additional information.

For the quick cell proliferation kits, the difference is that the ab65474 contains the stop solution as well, and the ab65472 not.

I hope this informationis helpful. Please do not hesitate to contact us again should you have any additional questions.

I wish you a great day as well :-)

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Dr. Thomas Weissensteiner

Verified customer

Submitted Oct 22 2009

Question
Answer

1) Crossreactivities: this antibody reacts with mouse E-selectin but not with human E-selectin. Other species not tested. 2)Blocking function: Ab2497 (20 microgram/ml) inhibited up to 70% of mouse PMN and up to 95% of human peripheral blood mononuclear cells adhesion to LPS-activated mlEND1 cells. For experimental conditions and figure see Hammel et al., Exp Cell Res 269: 266 (2001).

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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