Key features and details
- Rabbit polyclonal to CD62L
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CD62L antibody
See all CD62L primary antibodies
DescriptionRabbit polyclonal to CD62L
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Recombinant fragment corresponding to Human CD62L aa 39-372.
WTYHYSEKPMNWQRARRFCRDNYTDLVAIQNKAEIEYLEKTLPFSRSYYW IGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKEDCVEIYIKRNKD AGKWNDDACHKLKAALCYTASCQPWSCSGHGECVEIINNYTCNCDVGYYG PQCQFVIQCEPLEAPELGTMDCTHPLGNFSFSSQCAFSCSEGTNLTGIEE TTCGPFGNWSSPEPTCQVIQCEPLSAPDLGIMNCSHPLASFSFTSACTFI CSEGTELIGKKKTICESSGIWSNPSPICQKLDKSFSMIKEGDYNPLFIPV AVMVTAFSGLAFIIWLARRLKKGKKSKRSMNDPY
Database link: P14151
- IHC-P: Human tonsil tissue. WB: Human placenta tissue lysate; Jukat, CEM and HL-60 whole cell lysates; Rat and mouse spleen tissue lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
FormLyophilized:Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
After reconstitution as directed
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Tissue specificityExpressed in B cell lines and T lymphocytes.
Sequence similaritiesBelongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains.
- Information by UniProt
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD62L using ab264045 at 1 µg/ml in immunohistochemical analysis.
All lanes : Anti-CD62L antibody (ab264045) at 0.5 µg/ml
Lane 1 : Human placenta tissue lysate at 50 µg
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 40 µg
Lane 3 : CEM whole cell lysate at 40 µg
Lane 4 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 40 µg
Lane 5 : Rat spleen tissue lysate at 50 µg
Lane 6 : Mouse spleen tissue lysate at 50 µg
Predicted band size: 42 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264045 has not yet been referenced specifically in any publications.