Overview

  • Product name

    Anti-CD62L antibody - C-terminal
    See all CD62L primary antibodies
  • Description

    Rabbit polyclonal to CD62L - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 343-372 of Human CD62L.

  • Positive control

    • Jurkat cell line lysate; Human lymph tissue; Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab135792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • Tissue specificity

    Expressed in B cell lines and T lymphocytes.
  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A.11 antibody
    • AI528707 antibody
    • CD62 antigen ligand antibody
    • CD62 antigen-like family member L antibody
    • CD62L antibody
    • gp90-MEL antibody
    • IgA nephropathy, susceptibility to, included antibody
    • L Selectin antibody
    • L-selectin antibody
    • LAM-1 antibody
    • LAM1 antibody
    • LECAM1 antibody
    • LEU8 antibody
    • Leukocyte adhesion molecule 1 antibody
    • Leukocyte surface antigen Leu-8 antibody
    • Leukocyte-endothelial cell adhesion molecule 1 antibody
    • Lnhr antibody
    • LSEL antibody
    • Ly-22 antibody
    • Ly-m22 antibody
    • Lyam-1 antibody
    • LYAM1 antibody
    • LYAM1_HUMAN antibody
    • Lymph node homing receptor antibody
    • Lymphocyte adhesion molecule 1 antibody
    • Lymphocyte antigen 22 antibody
    • Lymphocyte surface MEL-14 antigen antibody
    • MEL-14 antibody
    • Pln homing receptor antibody
    • PLNHR antibody
    • Selectin L antibody
    • Selectin, lymphocyte antibody
    • SELL antibody
    • TQ1 antibody
    see all

Images

  • Flow cytometric analysis of Jurkat cells labelling CD62L with ab135792 (green line). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the antibody (ab135792, 1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody used was FITC conjugated Goat anti-Rabbit antibodies at 1/200 dilution for 40 min at room temperature. Blank control (blue line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph tissue labelling CD62L C-terminal with ab135792 at 1/25 dilution.  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-CD62L antibody - C-terminal (ab135792) at 1/1000 dilution + Jurkat whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 42 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Su S  et al. Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer. Cell Res 27:461-482 (2017). IHC ; Human . Read more (PubMed: 28290464) »
  • Kobawala TP  et al. Significance of TNF-a and the Adhesion Molecules: L-Selectin and VCAM-1 in Papillary Thyroid Carcinoma. J Thyroid Res 2016:8143695 (2016). IHC . Read more (PubMed: 26881177) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lymph nodes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate ph 6
Permeabilization
No
Specification
lymph nodes
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
70% ethanol-paraffin fixed

Miriam Valued Customer

Verified customer

Submitted Feb 01 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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