Overview

  • Product name

    Anti-CD62L antibody [DREG-56] (PE/Cy5®)
    See all CD62L primary antibodies
  • Description

    Mouse monoclonal [DREG-56] to CD62L (PE/Cy5®)
  • Host species

    Mouse
  • Conjugation

    PE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    PMA activated Human peripheral blood leukocytes.

  • Positive control

    • Human peripheral blood leukocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab95540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells.

ab67435 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • Tissue specificity

    Expressed in B cell lines and T lymphocytes.
  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A.11 antibody
    • AI528707 antibody
    • CD62 antigen ligand antibody
    • CD62 antigen-like family member L antibody
    • CD62L antibody
    • gp90-MEL antibody
    • IgA nephropathy, susceptibility to, included antibody
    • L Selectin antibody
    • L-selectin antibody
    • LAM-1 antibody
    • LAM1 antibody
    • LECAM1 antibody
    • LEU8 antibody
    • Leukocyte adhesion molecule 1 antibody
    • Leukocyte surface antigen Leu-8 antibody
    • Leukocyte-endothelial cell adhesion molecule 1 antibody
    • Lnhr antibody
    • LSEL antibody
    • Ly-22 antibody
    • Ly-m22 antibody
    • Lyam-1 antibody
    • LYAM1 antibody
    • LYAM1_HUMAN antibody
    • Lymph node homing receptor antibody
    • Lymphocyte adhesion molecule 1 antibody
    • Lymphocyte antigen 22 antibody
    • Lymphocyte surface MEL-14 antigen antibody
    • MEL-14 antibody
    • Pln homing receptor antibody
    • PLNHR antibody
    • Selectin L antibody
    • Selectin, lymphocyte antibody
    • SELL antibody
    • TQ1 antibody
    see all

Images

  • Staining of normal Human peripheral blood cells with PE-Cy5 Mouse IgG1 ? Isotype Control (open histogram) or ab95540 (filled histogram).
    Cells in the lymphocyte gate were used for analysis.

References

This product has been referenced in:

  • Jutila MA  et al. L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts. J Immunol 169:1768-73 (2002). WB ; Human . Read more (PubMed: 12165498) »
  • Kishimoto TK  et al. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc Natl Acad Sci U S A 87:2244-8 (1990). WB, Flow Cyt ; Human . Read more (PubMed: 2179952) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: Ficoll-Hypaque gradient isolation
Sample buffer: PBS, Ficoll-Hypaque
Permeabilization
No
Gating Strategy
lymphocytes

Abcam user community

Verified customer

Submitted Apr 09 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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