Product nameAnti-CD62L antibody [EPR17012-27]
See all CD62L primary antibodies
DescriptionRabbit monoclonal [EPR17012-27] to CD62L
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-Fr,IHC-P or IP
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD62L aa 150-350. The exact sequence is proprietary.
Database link: P18337
- WB: Mouse serum. Flow Cyt: Mouse splenocytes.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab253240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 75-100 kDa (predicted molecular weight: 42 kDa).|
FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Tissue specificityExpressed in B cell lines and T lymphocytes.
Sequence similaritiesBelongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains.
- Information by UniProt
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253240 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with anti-CD4 conjugated with Alexa Fluor® 647 and anti-CD44 conjugated with BV421. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.
Gated on viable CD4+ T cells.
All lanes : Anti-CD62L antibody [EPR17012-27] (ab253240) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse lymph node lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 75-100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 12165530).
Blocking/Dilution buffer: 5% NFDM/TBST.
ab253240 has not yet been referenced specifically in any publications.