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RecombinantRabMAb

Recombinant Anti-CD62L antibody [EPR17012-27] (ab253240)

  • Datasheet
  • Certificate of Compliance
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Abpromise

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Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)
  • Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)
  • Anti-CD62L antibody [EPR17012-27] (ab253240)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17012-27] to CD62L
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse

You may also be interested in

Protein
Product image
Recombinant Human CD62L protein (ab132972)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-CD62L antibody [EPR17012-27]
    See all CD62L primary antibodies
  • Description

    Rabbit monoclonal [EPR17012-27] to CD62L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Mouse CD62L aa 150-350. The exact sequence is proprietary.
    Database link: P18337

  • Positive control

    • WB: Mouse serum. Flow Cyt: Mouse splenocytes.
  • General notes

    ab253240 (EPR17012-27) is the capture of the ssELISA kit ab199085.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17012-27
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-CD62L antibody [EPR17012-27] - BSA and Azide free (ab254203)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD62L protein (ab132972)
  • Related Products

    • Mouse L-Selectin ELISA Kit (CD62L) (ab199085)

Applications

Our Abpromise guarantee covers the use of ab253240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75-100 kDa (predicted molecular weight: 42 kDa).
Flow Cyt 1/60.
  • Application notes
    Is unsuitable for ICC/IF,IHC-Fr,IHC-P or IP.
  • Target

    • Function

      Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
    • Tissue specificity

      Expressed in B cell lines and T lymphocytes.
    • Sequence similarities

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 2 Sushi (CCP/SCR) domains.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P14151 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 20343 Mouse
      • SwissProt: P18337 Mouse
      • Unigene: 1461 Mouse
      • Alternative names

        • A.11 antibody
        • AI528707 antibody
        • CD62 antigen ligand antibody
        • CD62 antigen-like family member L antibody
        • CD62L antibody
        • gp90-MEL antibody
        • IgA nephropathy, susceptibility to, included antibody
        • L Selectin antibody
        • L-selectin antibody
        • LAM-1 antibody
        • LAM1 antibody
        • LECAM1 antibody
        • LEU8 antibody
        • Leukocyte adhesion molecule 1 antibody
        • Leukocyte surface antigen Leu-8 antibody
        • Leukocyte-endothelial cell adhesion molecule 1 antibody
        • Lnhr antibody
        • LSEL antibody
        • Ly-22 antibody
        • Ly-m22 antibody
        • Lyam-1 antibody
        • LYAM1 antibody
        • LYAM1_HUMAN antibody
        • Lymph node homing receptor antibody
        • Lymphocyte adhesion molecule 1 antibody
        • Lymphocyte antigen 22 antibody
        • Lymphocyte surface MEL-14 antigen antibody
        • MEL-14 antibody
        • Pln homing receptor antibody
        • PLNHR antibody
        • Selectin L antibody
        • Selectin, lymphocyte antibody
        • SELL antibody
        • TQ1 antibody
        see all

      Images

      • Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)
        Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)

        Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253240 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

        Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with anti-CD4 conjugated with Alexa Fluor® 647 and anti-CD44 conjugated with BV421. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.

        Gated on viable CD4+ T cells.

      • Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)
        Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)
        All lanes : Anti-CD62L antibody [EPR17012-27] (ab253240) at 1/1000 dilution

        Lane 1 : Mouse serum
        Lane 2 : Mouse lymph node lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 42 kDa
        Observed band size: 75-100 kDa
        why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 12165530).

        Blocking/Dilution buffer: 5% NFDM/TBST.

      • Anti-CD62L antibody [EPR17012-27] (ab253240)
        Anti-CD62L antibody [EPR17012-27] (ab253240)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab253240? Please let us know so that we can cite the reference in this datasheet.

      ab253240 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab253240.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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