Recombinant Anti-CD62L antibody [EPR17012-27] (ab253240)
![Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)](https://www.abcam.com/ps/products/253/ab253240/Images/ab253240-2-253240FC1.jpg "Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17012-27] to CD62L
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD62L antibody [EPR17012-27]
See all CD62L primary antibodies -
Description
Rabbit monoclonal [EPR17012-27] to CD62L -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse CD62L aa 150-350. The exact sequence is proprietary.
Database link: P18337 -
Positive control
- WB: Mouse serum. Flow Cyt: Mouse splenocytes.
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General notes
ab253240 (EPR17012-27) is the capture of the ssELISA kit ab199085.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17012-27 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab253240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 75-100 kDa (predicted molecular weight: 42 kDa). | |
| Flow Cyt | 1/60. |
Target
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Function
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. -
Tissue specificity
Expressed in B cell lines and T lymphocytes. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 20343 Mouse
- SwissProt: P18337 Mouse
- Unigene: 1461 Mouse
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Alternative names
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
see all
Images
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Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)
Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253240 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with anti-CD4 conjugated with Alexa Fluor® 647 and anti-CD44 conjugated with BV421. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.
Gated on viable CD4+ T cells.
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![Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)](https://www.abcam.com/ps/products/253/ab253240/Images/ab253240-1-253240WB1.jpg)
Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)All lanes : Anti-CD62L antibody [EPR17012-27] (ab253240) at 1/1000 dilution
Lane 1 : Mouse serum
Lane 2 : Mouse lymph node lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 75-100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 12165530).
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-CD62L antibody [EPR17012-27] (ab253240)](https://www.abcam.com/ps/products/253/ab253240/Images/ab253240-3-benefits-of-recombinant-antibodies.png)
Anti-CD62L antibody [EPR17012-27] (ab253240)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab253240 has not yet been referenced specifically in any publications.
