Recombinant
RabMAb

Recombinant Anti-CD62L antibody [EPR17012-27] (ab253240)

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Overview

  • Product name

    Anti-CD62L antibody [EPR17012-27]
    See all CD62L primary antibodies

  • Description

    Rabbit monoclonal [EPR17012-27] to CD62L

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-Fr,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment within Mouse CD62L aa 150-350. The exact sequence is proprietary.
    Database link: P18337

  • Positive control

    • WB: Mouse serum. Flow Cyt: Mouse splenocytes.

  • General notes

    ab253240 (EPR17012-27) is the capture of the ssELISA kit ab199085.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab253240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75-100 kDa (predicted molecular weight: 42 kDa).
Flow Cyt 1/60.
  • Application notes
    Is unsuitable for ICC/IF,IHC-Fr,IHC-P or IP.

    • Target

    • Function

      Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.

    • Tissue specificity

      Expressed in B cell lines and T lymphocytes.

    • Sequence similarities

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 2 Sushi (CCP/SCR) domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • A.11 antibody
        • AI528707 antibody
        • CD62 antigen ligand antibody
        • CD62 antigen-like family member L antibody
        • CD62L antibody
        • gp90-MEL antibody
        • IgA nephropathy, susceptibility to, included antibody
        • L Selectin antibody
        • L-selectin antibody
        • LAM-1 antibody
        • LAM1 antibody
        • LECAM1 antibody
        • LEU8 antibody
        • Leukocyte adhesion molecule 1 antibody
        • Leukocyte surface antigen Leu-8 antibody
        • Leukocyte-endothelial cell adhesion molecule 1 antibody
        • Lnhr antibody
        • LSEL antibody
        • Ly-22 antibody
        • Ly-m22 antibody
        • Lyam-1 antibody
        • LYAM1 antibody
        • LYAM1_HUMAN antibody
        • Lymph node homing receptor antibody
        • Lymphocyte adhesion molecule 1 antibody
        • Lymphocyte antigen 22 antibody
        • Lymphocyte surface MEL-14 antigen antibody
        • MEL-14 antibody
        • Pln homing receptor antibody
        • PLNHR antibody
        • Selectin L antibody
        • Selectin, lymphocyte antibody
        • SELL antibody
        • TQ1 antibody
        see all

      Images

      • Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)
        Flow Cytometry - Anti-CD62L antibody [EPR17012-27] (ab253240)

        Flow cytometric analysis of mouse splenocytes labeling CD62L with ab253240 at 1/60 (Right panel) compared with a Rabbit monoclonal IgG (ab172730) (Left panel). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

        Cells were stained with rabbit IgG (Left) or anti-CD62L RabMab (Right). Then stained with anti-CD4 conjugated with Alexa Fluor® 647 and anti-CD44 conjugated with BV421. While CD4+ splenocytes express CD62L and CD44, their expressions are mutually exclusive.

        Gated on viable CD4+ T cells.

      • Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)
        Western blot - Anti-CD62L antibody [EPR17012-27] (ab253240)
        All lanes : Anti-CD62L antibody [EPR17012-27] (ab253240) at 1/1000 dilution

        Lane 1 : Mouse serum
        Lane 2 : Mouse lymph node lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

        Predicted band size: 42 kDa
        Observed band size: 75-100 kDa
        why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 12165530).

        Blocking/Dilution buffer: 5% NFDM/TBST.

      References

      ab253240 has not yet been referenced specifically in any publications.

      Publishing research using ab253240? Please let us know so that we can cite the reference in this datasheet.

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