Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22188-297] to CD62L - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Rat
Product nameAnti-CD62L antibody [EPR22188-297] - BSA and Azide free
See all CD62L primary antibodies
DescriptionRabbit monoclonal [EPR22188-297] to CD62L - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or WB
Species reactivityReacts with: Rat
Recombinant full length protein within Rat CD62L aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q63762
- Flow Cyt: Rat primary splenocytes.
Ab245124 is the carrier-free version of ab218829. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab245124 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab245124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
RelevanceCD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
Cellular localizationCell Membrane; single-pass type I membrane protein.
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
Flow cytometric analysis of rat primary splenocytes labeling CD62L with ab218829 at 1/500 dilution (top right panel), compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Upper left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Top panel: Cells were stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left) or anti-CD62L RabMab (Right). Data shown was gated on viable cells.
Down regulation of CD62L on activated T cells (CD3+FSChigh, blue) compared to Naïve T cells (CD3+CD62L+, red) was shown on the bottom panel
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218829).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245124 has not yet been referenced specifically in any publications.