Recombinant Anti-CD62L antibody [EPR22188-297] - BSA and Azide free (ab245124)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22188-297] to CD62L - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Rat
Related conjugates and formulations
Overview
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Product name
Anti-CD62L antibody [EPR22188-297] - BSA and Azide free
See all CD62L primary antibodies -
Description
Rabbit monoclonal [EPR22188-297] to CD62L - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or WB -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Rat primary splenocytes.
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General notes
ab245124 is the carrier-free version of ab218829.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22188-297 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Relevance
CD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins. -
Cellular localization
Cell Membrane; single-pass type I membrane protein. -
Database links
- Entrez Gene: 29259 Rat
- SwissProt: Q63762 Rat
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Alternative names
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
see all
Images
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Flow cytometric analysis of rat primary splenocytes labeling CD62L with ab218829 at 1/500 dilution (top right panel), compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Upper left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Top panel: Cells were stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left) or anti-CD62L RabMab (Right). Data shown was gated on viable cells.
Down regulation of CD62L on activated T cells (CD3+FSChigh, blue) compared to Naïve T cells (CD3+CD62L+, red) was shown on the bottom panel
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218829).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab245124 has not yet been referenced specifically in any publications.