Anti-CD62L antibody [OX85] (ab238473)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD62L antibody [OX85]
See all CD62L primary antibodies -
Description
Mouse monoclonal [OX85] to CD62L -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Fusion protein corresponding to Rat CD62L (extracellular). Fused at the C-terminal end to domains 3 and 4 of rat CD4.
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Positive control
- Flow Cyt: Lewis rat splenocytes.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OX85 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab238473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use a concentration of 0.2 µg/ml. |
Target
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Function
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. -
Tissue specificity
Expressed in B cell lines and T lymphocytes. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 29259 Rat
- SwissProt: P30836 Rat
- Unigene: 10461 Rat
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Alternative names
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
see all
Images
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![Flow Cytometry - Anti-CD62L antibody [OX85] (ab238473)](https://www.abcam.com/ps/products/238/ab238473/Images/ab238473-1-ab238473flow1.jpg)
Flow Cytometry - Anti-CD62L antibody [OX85] (ab238473)LabLewis rat splenocytes stained with ab238473 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab238473) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
Datasheets and documents
References
ab238473 has not yet been referenced specifically in any publications.
